大黄鱼鱼卵磷脂与乳清分离蛋白复合分散液的制备工艺研究  

Preparation of the composite dispersion of large yellow croaker roe phospholipid and whey isolate protein

作　　者：林艺璇 陈宇涵 李响 陈继承[1] 梁鹏[1] LIN Yixuan;CHEN Yuhan;LI Xiang;CHEN Jicheng;LIANG Peng(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002

出　　处：《河南工业大学学报（自然科学版）》2023年第3期18-24,56,共8页Journal of Henan University of Technology：Natural Science Edition

基　　金：福建省自然科学基金项目(2021J01109);福建省财政专项补助项目(KJG19009A)。

摘　　要：大黄鱼鱼卵磷脂(LYCRPLs)富含DHA和EPA等多不饱和脂肪酸,并具有良好的乳化性质。为了拓宽LYCRPLs在乳制品中的应用,采用高速均质法将其与乳清分离蛋白(WPI)复配制备分散液,以粒径和澄清指数作为分散液稳定性的考察指标,在单因素试验的基础上进行响应面试验优化分散液的制备工艺条件。结果表明:影响分散液稳定性的因素由大到小为pH值、LYCRPLs添加量、WPI添加量、均质速度、均质时间;分散液的最佳制备工艺为均质速度23 000 r/min、WPI添加量1.0%、LYCRPLs添加量0.50%,此条件下制备的分散液粒径为(215.20±2.12) nm,澄清指数为0.080±0.003,该分散液储藏稳定性良好。本研究为LYCRPLs的高值利用提供了理论依据。Large yellow croaker roe phospholipid(LYCRPLs)is rich in polyunsaturated fatty acids such as DHA and EPA,and has high emulsification properties.To broaden the application value of LYCRPLs,the dispersions were prepared by employing a high-speed homogenization method with the utilization of LYCRPLs and Whey isolate protein(WPI).This study investigated the effect of pH,homogenization time,homogenization speed,WPI addition and LYCRPLs addition on the stability of LYCRPLs-WPI dispersions.On the basis of the single factor experiments,the particle size and clarification index of the dispersions were used as the response values,and the three factors with a greater influence on stability were selected to design the response surface analysis method.LYCRPLs-WPI dispersions were prepared under optimal process conditions and stored at temperature(25℃)for 28 d.The particle size,PDI and Zeta potential of the dispersions were measured every 7 d.The results showed that the influence factors on the stability of the dispersions were in this order:pH value>LYCRPLs addition>WPI addition>homogenization speed>homogenization time.Combined with the actual situation,the optimized condition for preparing the dispersions was as follows:23000 r/min homogenization speed,1.0%WPI addition and 0.50%LYCRPLs addition,and under this condition,the particle size of the prepared dispersion was(215.20±2.12)nm and the clarification index was 0.080±0.003 as measured by three replicate experiments,which was close to the theoretical results,indicating that the model works well.Meanwhile,at the beginning of storage(0 d),the LYCRPLs-WPI dispersions were small in size,with a large absolute potential value and a more uniform distribution.In addition,the PDI values did not change significantly during the storage period.The results show excellent storage stability of the dispersion.These results provide a theoretical basis for the high value utilization LYCRPLs.

关 键 词：大黄鱼鱼卵 磷脂 乳清分离蛋白 响应面法 稳定性 

分 类 号：TS201.2[轻工技术与工程—食品科学]