不同种类乳杆菌单独及协同咪康唑应用对白假丝酵母菌生长影响的体外研究  

In vitro study of the effect of different Lactobacillus alone and combination with miconazole on the growth of Pseudomonas albicans

作　　者：杜梦瑶 赵晓雯 尚翔 王和舒琦 白会会 范琳媛 宗晓楠 张展 李婷 刘朝晖 Du Mengyao;Zhao Xiaowen;Shang Xiang(Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing Maternal and Child Health Care Hospital,Beijing 100026;Capital Medical University,Beijing 100069)

机构地区：[1]首都医科大学附属北京妇产医院/北京妇幼保健院,北京100026 [2]首都医科大学,北京100069

出　　处：《现代妇产科进展》2023年第7期488-493,共6页Progress in Obstetrics and Gynecology

摘　　要：目的:研究不同种类乳杆菌单独及协同咪康唑应用对白假丝酵母菌生长状态的影响。方法:选取健康女性阴道分泌物中分离纯化鉴定的卷曲、詹氏、加氏、约氏、嗜酸乳杆菌和临床常用乳杆菌活菌制剂分离纯化的德氏乳杆菌共6种,与白假丝酵母菌标准株共培养。观察不同种类乳杆菌与白假丝酵母菌共培养24h的生长情况及生物膜形成情况。比较乳杆菌单独应用及乳杆菌与咪康唑共同作用于白假丝酵母菌的菌落生长情况。结果:6种乳杆菌与白假丝酵母菌共培养后,白假丝酵母菌菌落数均减少,其中约氏乳杆菌抑制真菌能力最强,德式乳杆菌次之,嗜酸乳杆菌最弱。高浓度的德氏乳杆菌抑菌效果最好,真菌存活率低。约氏、嗜酸乳杆菌在24h时均可形成生物膜,其中约氏乳杆菌成膜能力较嗜酸乳杆菌强,约氏、嗜酸乳杆菌均对白假丝酵母菌生物膜有一定破坏作用,其中约氏乳杆菌破坏能力比嗜酸乳杆菌强。6种乳杆菌分别和不同浓度咪康唑联合应用与白假丝酵母菌共培养,达到相同抑菌效果时需要的药物浓度减少(P<0.05)。结论:不同乳杆菌对白假丝酵母菌的生长及生物膜形成均有一定抑制作用,但需要较大浓度,乳杆菌与咪康唑有协同抑制白假丝酵母菌的作用。Objective:To study the effect of different Lactobacillus vaginalis alone and synergistic miconazole applications on the growth status of Pseudomonas albicans.Methods:A total of 6 species of Lactobacillus crispatus,Lactobacillus jensenii,Lactobacillus gasseri,Lactobacillus johnsonii,Lactobacillus acidophilus and Lactobacillus delbrueckii was isolated and purified from the vaginal secretions of healthy women were selected and co-cultured with the standard strain of Pseudomonas albicans to observe the growth and biofilm formation of different species of Lactobacillus and Pseudomonas albicans in co-culture for 24h.The colony growth of Lactobacillus alone and Lactobacillus with miconazole was compared with that of Pseudomonas albicans.Results:After co-culture of six Lactobacillus species with Pseudomonas albicans,the number of Pseudomonas albicans colonies decreased,with Lactobacillus johnsonii being the strongest in inhibiting the fungus,followed by Lactobacillus delbrueckii.Lactobacillus acidophilus was the weakest.High concentrations of Lactobacillus Delbruck had the best inhibitory effect and low fungal survival.Lactobacillus johnsonii and Lactobacillus acidophilus can both form biofilms at 24h,with Lactobacillus johnsonii having a stronger film-forming ability than Lactobacillus acidophilus.Six species of Lactobacillus were co-cultured with miconazole and Pseudomonas albicans,respectively,and the drug concentrations required to achieve the same inhibitory effect were reduced(P<0.05).Conclusion:The different Lactobacillus species all had an inhibitory effect on the growth and biofilm formation of Pseudomonas albicans but required larger concentrations,and the six Lactobacillus species studied differed in their ability to inhibit the fungus.Lactobacillus and miconazole had a synergistic effect in inhibiting Pseudomonas albicans.

关 键 词：乳杆菌 外阴阴道假丝酵母菌病 共培养 生物膜 咪康唑 

分 类 号：R711.72[医药卫生—妇产科学]