甘蓝型油菜WOX基因家族的鉴定与表达分析  

Identification and Expression Analysis of WOX Gene Family in Brassica napus

作　　者：陈玲 王旺田[1] 蒲媛媛[1] 方彦[1] 武军艳[1] 马骊[1] 李学才[1] 刘丽君 陈常兵 王积军 孙万仓[1] CHEN Ling;WANG Wangtian;PU Yuanyuan;FANG Yan;WU Junyan;MA Li;LI Xuecai;LIU Lijun;CHEN Changbing;WANG Jijun;SUN Wancang(Agronomy College,Gansu Agricultural University,State Key Laboratory of Aridland Crop Science,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学农学院,省部共建干旱生境作物学国家重点实验室,兰州730070

出　　处：《西北农业学报》2023年第8期1173-1186,共14页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31860388,31960435);甘肃省科技重大专项(22ZD6NA009);2022年现代丝路寒旱农业科技支撑项目(GSLK-2022-9);国家油菜产业技术体系(GARS-12)。

摘　　要：对甘蓝型油菜WOX基因家族进行研究,利用生物信息学方法鉴定到58个家族成员,对其理化性质、系统发育树、基因结构、保守结构域、染色体位置、顺式作用元件和表达模式等进行分析,结果表明:氨基酸长度为121~397,分子质量为13962.77~44157.46 u,等电点为5.23~9.63,亲水性均小于0,不稳定系数为42.12~75.63,亚细胞定位在细胞核和叶绿体;系统进化树将WOX基因分为3大类和9个亚支;相同亚支的成员motif和基因结构相似或相同;除BnWOX1、BnWOX3、BnWOX4、BnWOX15、BnWOX32、BnWOX42、BnWOX43、BnWOX51、BnWOX53基因外,其余49个BnWOX基因不均匀分布在19条染色体中的18条染色体上(ChrC06除外),有38对串联重复基因;基因上游序列中包含转录因子结合位点、逆境响应、光响应、激素响应、分生组织调控等顺式作用元件等;各组织器官表达分析表明,BnWOX基因在根、茎尖、种子、角果中表达量较高;对WOX基因进行定量分析发现,在冷胁迫和干旱胁迫下基因表达量有显著变化,表明BnWOX基因可能在油菜响应冷/旱胁迫的调控机制中发挥着重要作用。In this study,the WOX gene family of Brassica napus was studied,and 58 family members were identified by bioinformatics methods,their physicochemical properties,phylogenetic tree,gene structure,conserved domains,chromosomal locations,cis-acting elements and expression patterns were analyzed.The results showed that the amino acid length was 121-397,molecular mass was 13962.77-44157.46 u,isoelectric point was 5.23-9.63,hydrophilicity was less than 0,instability coefficient was between 42.12-75.63,subcellular localization was in nucleus and chloroplast;the WOX genes were divided into three major groups and nine subclades in the phylogenetic tree;members of the same subclade had similar or identical motifs and gene structures;except forBnWOX1,BnWOX3,BnWOX4,BnWOX15,BnWOX32,BnWOX42,BnWOX43,BnWOX51,BnWOX53genes.49 WOX genes were unevenly distributed in 18 of 19 chromosomes(except ChrC06),and 38 tandem repeats were found;the upstream sequences of the genes included transcription factor binding sites,stress response,light response,hormone response,meristem regulation and other cis-acting elements,etc.Organ expression analysis showed that BnWOX gene was highly expressed in roots,shoot tips,seeds and siliques;after quantitative analysis of BnWOX genes,it was found that the gene expression changed significantly under cold stress and drought stress,it indicated that BnWOX gene may play an important role in the regulatory mechanism of rapeseed response to cold/drought stress.

关 键 词：甘蓝型油菜 WOX基因家族 系统进化 非生物胁迫 

分 类 号：S565.4[农业科学—作物学]