快速溶剂萃取-电雾式检测器同时测定山楂及其炮制品中5种三萜酸类成分的含量  

Simultaneous determination of five triterpenic acids in Crataegi Fructus by accelerated solvent extraction-charged aerosol detector

作　　者：杜蕾蕾[1] 陈雏[2] 冉良骥 DU Leilei;CHEN Chu;RAN Liangji(College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China;Sichuan Key Laboratory of Traditional Chinese Medicine Quality and Innovation,Sichuan Academy of Traditional Chinese Medicine,Chengdu,610041,China;Thermo Fisher Scientific(China)Co.,Ltd.,Shanghai,201206,China)

机构地区：[1]成都中医药大学民族医药学院,成都611137 [2]四川省中医药科学院中药材品质及创新中药研究四川省重点实验室,成都610041 [3]赛默飞世尔科技(中国)有限公司,上海201206

出　　处：《环境化学》2023年第6期2132-2135,共4页Environmental Chemistry

摘　　要：建立一种快速溶剂萃取-电雾式检测器(ASE-CAD)同时测定山楂及其炮制品中5种三萜酸类成分含量的方法.试验确定ASE萃取山楂的最优条件:以甲醇作溶剂,提取温度100℃,静态提取时间5 min,循环次数2次,提取池冲洗体积60%.HPLC方法:采用Thermo Acclaim C30(4.6 mm×250 mm,5μm)色谱柱,乙腈-1%乙酸铵为流动相,梯度洗脱,体积流量1.0 mL·min^(-1),柱温25℃,CAD雾化温度70℃,采集频率5 Hz,分别测定齐墩果酸、熊果酸、白桦脂酸、科罗索酸、山楂酸的含量.结果表明,齐墩果酸、白桦脂酸、科罗索酸、山楂酸的线性范围为5—75μg·mL^(-1),熊果酸的线性范围为5—250μg·mL^(-1);相关系数均不小于0.9970;平均回收率在88%—101%之间,RSD均小于5%.该方法准确、简便、重复性好,适用于山楂及其炮制品中齐墩果酸、熊果酸、白桦脂酸、科罗索酸、山楂酸的含量测定.The objective of this paper is to establish a method for the simultaneous determination of five triterpenic acids in Crataegi Frucuts and its processed products by accelerate solvent extraction(ASE)-charged aerosol detector(CAD).The optimal extraction conditions were determined by the ASE test:methanol was used as solvent,ASE extraction temperature was 100℃,extraction time was 5 min and cycle time was 2.For the HPLC analysis,Thermo Acclaim C30(4.6 mm×250 mm,5μm)was employed,the mobile phase for the analysis pump was composed of acetonitrile-1%ammonium acetate in program of gradient elution,and the flow rate was 1.0 mL·min with oven temperature 25℃.The parameters applied for CAD are nebulizer temperature at 70℃and data rate at 5 Hz.The results showed that the calibration curves were linear over a concentration range of 5—75μg·mL^(-1) for oleanolic acid,betulinic acid,corosolic acid and maslinic acid,and 5—250μg·mL^(-1) for ursolic acid.The correlation coefficients were greater than 0.9970.The average recoveries were between 88%and 101%,with RSDs less than 5%.This method is accurate,simple and reproducible.It can be used for the determination of oleanolic acid,betulinic acid,corosolic acid,maslinic acid and ursolic acid in Crataegi Frucuts.

关 键 词：山楂 三萜酸 快速溶剂萃取 电雾式检测器 炮制品 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O652[轻工技术与工程—食品科学与工程]