Novel potential XOD inhibitory peptides derived from Trachinotus ovatus:Isolation,identification and structure-function analysis  

作　　者：Mengfan Hou Huan Xiang Xiao Hu Shengjun Chen Yanyan Wu Jiachao Xu Xianqing Yang 

机构地区：[1]Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou,510300,China [2]Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang,222005,China [3]College of Food Science and Engineering,Ocean University of China,Qingdao,266003,China [4]Collaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing,Dalian,116034,China

出　　处：《Food Bioscience》2022年第3期383-393,共11页食品生物科学（英文）

基　　金：supported by the Key-Area Research and Development Program of Guangdong Province(2020B1111030004);the Guangdong Basic and Applied Basic Research Foundation(2019A1515011588);the Pearl River S&T Nova Program of Guangzhou(201906010081);the China Agriculture Research System of MOF and MARA(CARS-47);the Special Scientific Research Funds for Central Non-profit Institutes,Chinese Academy of Fishery Sciences(2020TD69);the Central Public-interest Scientific Institution Basal Research Funds,South China Sea Fisheries Research Institute,CAFS(2021SD06);Qingdao Science and technology benefit people special project(20-3-4-31-nsh).

摘　　要：This study aimed to isolate and identify the novel potential Xanthine oxidase(XOD)inhibitory peptides derived from Trachinotus ovatus hydrolysate(TOH)(hydrolysis by neutral protease),and investigate their structure-function relationship.Initially,TOH was separated to four fractions(F1–F4)by gel filtration chromatography and F4 was chosen with higher XOD inhibitory rate(85.16%±0.566,25 mg/mL)and lower molecular weight(95.73%,<1000 Da).F4 was further characterized by reversed-phase high performance liquid chromatography(RP-HPLC)coupled with Nano-HPLC-MS/MS and four peptides(FPAW,LLPW,WLLP and FHLP)were identified with IC50 value of 3.81±0.18 mM,4.17±0.12 mM,43.06±0.73 mM,and over 50.00 mM respectively.It can be concluded that Trp-containing peptides especially located in C-terminal could inhibit XOD effectively.Moreover,the XOD-inhibiting actions of these peptides involvedπ-πstacking(Phe914 and Phe1009 in XOD),hydrogen bonding via molecular docking.Lineweaver-Burk equation showed the inhibition type of FPAW was a mixed inhibition.Circular dichroism(CD)spectra indicated FPAW could combine and change the secondary structure of XOD.Therefore,two peptides of FPAW and LLPW might have potential to be used to alleviate hyperuricemia in the future.

关 键 词：Trachinotus ovatus XOD inhibitory Activity Trp-containing peptides Nano-HPLC-MS/MS Molecular docking 

分 类 号：TS254[轻工技术与工程—水产品加工及贮藏工程]