In vitro assessment of the effect of microencapsulation techniques on the stability,bioaccessibility and bioavailability of mulberry leaf bioactive compounds  

作　　者：William Tchabo Giscard Kuate Kaptso Guillaume Legrand Ngolong Ngea Kenuo Wang Guifeng Bao Yongkun Ma Xiangyang Wang Carl Moses Mbofung 

机构地区：[1]School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou,310018,PR China [2]Department of Food Science and Nutrition,National Advanced School of Agro-Industrial Sciences (ENSAI),University of Ngaoundere,PO Box 455,Ngaoundere,Cameroon [3]Department of Social Economy and Family Management,Higher Technical Teacher's Training College (HTTTC),University of Buea,P.O Box 249 Buea Road,Kumba South West Region,Cameroon [4]Department of Chemical Engineering,School of Chemical Engineering and Mineral Industries (EGCIM),University of Ngaoundere,PO Box 454,Ngaoundere,Cameroon [5]Institute of Fisheries Sciences,University of Douala,PO Box 24157,Douala,Cameroon [6]School of Food and Biological Engineering,Jiangsu University,301 Xuefu Road,Zhenjiang,212013,PR China

出　　处：《Food Bioscience》2022年第3期450-462,共13页食品生物科学（英文）

摘　　要：Mulberry(Morus alba L.)leaf extract is well-known for its health-promoting features.However,food processing conditions affect its bioactive profile,which could be limited by encapsulation.Nevertheless,assessing the impact of encapsulating techniques on the digestibility of embedded biocompounds requires further knowledge.Hence,the aimed of this study was to assess the effects of carrier materials and drying techniques on(i)bioaccessibility and bioavailability indexes,(ii)stability of nutraceuticals,and(iii)changes in antioxidant activities of mulberry leaf extract powder by using an in vitro oral-gastrointestinal digestion.Digestion was performed out at 37°C in darkness by shaking encapsulated extract with human saliva during 2 min,followed by 2 h of incubation with a pepsin-HCl mixture,then mixed for 2 h with bile salts and pancreatin.After digestion the samples were acidified and HPLC assayed.A significant(p<0.05)decrease in nutraceuticals content was noted during the intestinal phase with more reduction in gamma-aminobutyric acid(34.91–51.14%)compared to that of flavonols(16.58–28.90%),1-deoxynojirimycin(17.56–20.42%)and phenolic acids(0.53–0.67%)in the gastric digesta.Although encapsulation was observed to enhance the bioaccessibility,negative effects were found in terms of the bioefficiency and bioavailability.Furthermore,encapsulation techniques mostly affected the ion reducing capacity than the radical scavenging capacity.The outcomes suggested that carriers had a major effect on the digestibility and antioxidative activity,whilst the drying techniques mostly affect the bioaccessibility and bioavailability of the flavonols.

关 键 词：Morus alba L. Antioxidant activity MALTODEXTRIN Sodium carboxymethyl cellulose Spray dry Freeze dry 

分 类 号：O62[理学—有机化学]