Antibacteria activities of phenolics from"Jinshiji"thinned-young apple on halitosis-related bacteria  

作　　者：Ting Liu Tian Gong Furong Wang Xueru Zhou Yali Yang Yurong Guo 

机构地区：[1]College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an,PR China [2]National Research and Development Center of Apple Processing Technology,PR China

出　　处：《Food Bioscience》2022年第3期793-801,共9页食品生物科学（英文）

基　　金：supported by the National Natural Science Foundation of China(No.31701563);the Key Research and Development Program of Shaanxi Province(No.2019NY-124);Ministry of Education(XGZX 2021G08);the Fundamental Research Funds for the Central Universities at Shaanxi Normal University(1301031057).

摘　　要：This article aims to explain the inhibitory mechanism of thinned-young apple polyphenols(YAP)toward Fusobacterium nucleatum(F.nucleatum),Porphyromonas gingivalis(P.gingivalis)and Prevotella intermedia(P.intermedia).YAP has optimal antibacterial concentration against F.nucleatum(10.00 mg/mL),P.gingivalis(8.00 mg/mL)and P.intermedia(8.00 mg/mL),with viability of 47.97%,38.01%and 36.94%,respectively.Scanning electron microscopy(SEM)and confocal laser scanning microscopy(CLSM)observation revealed that YAP could cause the morphological changes of the halitosis-related bacterial cells and induce cell apoptosis.With the increase of YAP treatment concentrations,the permeability of the outer membranes(OM)and inner membranes(IM)of halitosis-related bacteria dramatically increased,resulting in the release of proteins and nucleic acids.In addition,bacterial cell membrane potential(MP)decreased after exposure to YAP.The results of this study provide new antibacterial agents for halitosis-related bacteria and find a new way for the development of thinned-young apples.

关 键 词：Thinned-young apple polyphenols HALITOSIS Cell membrane F.nucleatum P.gingivalis P.intermedia 

分 类 号：TS201.3[轻工技术与工程—食品科学]