鸭源性H6N1亚型禽流感病毒在猪和人呼吸道的复制及其基因特点  

Replication in the swine and human respiratory tract and genetic characteristics of duck-originated H6N1 subtype avian influenza virus

作　　者：解中 钟伟娟 张增峰 Xie Zhong;Zhong Weijuan;Zhang Zengfeng(Department of Microbiology,School of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学基础医学院微生物学教研室,南宁530021

出　　处：《广西医科大学学报》2023年第4期618-623,共6页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.31660041);广西自然科学基金资助项目(No.2018GXNSFAA281300)。

摘　　要：目的:探索鸭源性H6N1亚型禽流感病毒是否可在人以及流感病毒的重要中间宿主猪中有效复制、感染。方法:选择前期分离的2株鸭源性H6N1病毒DK/Jiangxi/35634/2016、DK/Guiyang/2462/2007,受体结合特异性法分析病毒的受体结合特性,全基因组测序分析病毒基因位点的变化,体外感染人和猪呼吸道组织。结果:2株鸭源性H6N1病毒均与禽型SAα-2,3Gal受体结合,基因测序分析显示HA裂解位点为PQIETR/GL,属低致病性禽流感病毒的分子特征,HA、NA、PB2等主要基因位点未发生突变,但病毒可在人肺组织的肺泡细胞和巨噬细胞、猪气管上皮细胞以及肺组织的肺泡细胞和巨噬细胞增殖并引起细胞损伤、组织结构的破坏。结论:鸭源性H6N1亚型禽流感病毒具有禽流感病毒的基因特点,虽然仍与禽型SAα-2,3Gal受体结合,但已具有跨种间屏障感染猪和人的能力。因此,必须密切监测家禽中流感病毒H6N1流行和进化演变。Objective:To explore whether duck-originated H6N1 subtype avian influenza virus was effectively replicable and infectious in humans and swines.Methods:Two strains of duck-originated H6N1 virus DK/Jiangxi/35634/2016 and DK/Guiyang/2462/2007 isolated in the previous period were selected,the receptor-binding characteristics of the viruses were analyzed by receptor binding specificity method,the changes of of viral gene loci were analyzed by whole genome sequencing,and the respiratory tract tissues of humans and swines were infected in vitro.Results:Both strains of H6N1 virus were bound to avian SAα-2,3Gal receptors.Gene sequencing results showed that the hemagglutinin(HA)cleavage locus was PQIETR/GL,belonging to the molecular characteristics of low-pathogenic AIV influenza virus,HA,NA,PB2 and other major gene loci were not mutated,but the two strains of virus could proliferate in alveolar cells and macrophages of human lung tissues,as well as in tracheal epithelial cells,alveolar cells and macrophages of swine lung tissues,and cause cell and tissue structure damages.Conclusion:Duck-originated H6N1 subtype avian influenza virus has the genetic characteristics of avian influenza virus.Although it still binds to avian SAα-2,3Gal receptors,it has the ability to infect swines and humans across the interspecies barrier.Therefore,the epidemic and evolution of influenza virus H6N1 in poultry should be closely monitored.

关 键 词：禽流感病毒 H6N1亚型 基因特点 呼吸道组织 

分 类 号：R373[医药卫生—病原生物学]