层次分析-熵权法结合响应面法优化五味子藤茎木脂素超声提取工艺  

Optimization of ultrasonic extraction process of lignans from Schisandra chinensis vine stem by analytic hierarchy process-entropy weight method combined with response surface methodology

作　　者：石畅 高畅 郭晓瑞 王洪政[1,2,3] 徐明远 刘岩[4] 张晶 邢凯鑫 唐中华[1,2,3] SHI Chang;GAO Chang;GUO Xiao-Rui;WANG Hong-Zheng;XU Ming-Yuan;LIU Yan;ZHANG Jing;XING Kai-Xin;TANG Zhong-Hua(Key Laboratory of Plant Ecology,Northeast Forestry University,Harbin 150040,China;College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040,China;Heilongjiang Key Laboratory of Ecological Utilization of Forest Derived Active Substances,Harbin 150040,China;School of Clinical Medicine,Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;Heilongjiang Academy of Forestry,Harbin 150040,China;Heilongjiang Provincial Natural Resources Rights and Interests Investigation and Monitoring Institute,Harbin 150080,China)

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040 [2]东北林业大学化学化工与资源利用学院,哈尔滨150040 [3]黑龙江省林源活性物质生态利用重点实验室,哈尔滨150040 [4]黑龙江中医药大学临床医学院,哈尔滨150040 [5]黑龙江省林业科学院,哈尔滨150040 [6]黑龙江省自然资源权益调查监测院,哈尔滨150080

出　　处：《食品安全质量检测学报》2023年第12期293-302,共10页Journal of Food Safety and Quality

基　　金：科技部国家科技基础资源调查计划项目(2019FY100500);黑龙江省博士后基金面上项目(LBH-Z20091);黑龙江省中医药管理局中医药科研项目(ZHY-2022-138);黑龙江省自然科学基金联合引导项目(LH2022H084);黑龙江省中医药经典普及化专项课题项目(ZYW2022-059)。

摘　　要：目的采用响应面法优化五味子藤茎木脂素超声提取工艺。方法以采用层次分析-熵权法计算的7种木脂素含量的综合评分为指标,以液料比、乙醇体积分数、超声时间3个因素进行单因素实验,结合响应面设计优化五味子藤茎木脂素的超声提取工艺,并考察提取液的抗氧化活性。结果响应面优化结果显示,液料比、乙醇体积分数、超声时间对木脂素综合评分均有显著的影响(P<0.05),影响强度依次为:乙醇体积分数>液料比>超声时间。五味子藤茎木脂素的最佳提取工艺为:液料比20(mL/g)、乙醇体积分数75%、提取时间40 min,在此条件下的综合评分为99.21,与模型预测数值相差较小,提取液具有较好的抗氧化作用。结论在最优条件下,五味子藤茎木脂素提取率较高且具有抗氧化药理活性。Objective To optimize the ultrasonic extraction process of lignans from Schisandra chinensis vine stem using response surface methodology.Methods The comprehensive score of 7 kinds of lignans content calculated by the analytic hierarchy process and entropy weight method was used as the index.Single-factor experiment was conducted with 3 factors,including liquid-to-material ratio,the volume fraction of ethanol,and ultrasonic time.The response surface design was used to optimize the ultrasonic extraction process of lignans from the vine stem,and the antioxidant activity of the extract was investigated.Results The response surface optimization results showed that the liquid-to-material ratio,volume fraction of ethanol,and ultrasonic time had significant effects on the comprehensive score of lignans(P<0.05),and the strength of influence was in the following order:Ethanol volume fraction>liquid-to-material ratio>ultrasonic time.The optimal extraction process of lignans from stems of Schisandra chinensis was as follows:Liquid-solid ratio of 20(mL/g),ethanol volume fraction of 75%,extraction time of 40 min.Under these conditions,the comprehensive score was 99.21,which was little different from the predicted value of the model,and the extract had a good antioxidant effect.Conclusion Under the optimal conditions,the extraction rate of lignans from the vine stem of Schisandra chinensis is high and has antioxidant activity.

关 键 词：五味子 层次分析法 熵权法 响应面法 超声提取法 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]