Impact of interstitial cells of Cajal on slow wave and gallbladder contractility in a guinea pig model of acute cholecystitis  

作　　者：Fan Ding Run Guo Fang Chen Li-Ping Liu Zheng-Yu Cui Yi-Xing Wang Gang Zhao Hai Hu 

机构地区：[1]Center of Gallbladder Disease,Shanghai East Hospital,School of Medicine,Tongji University,Shanghai 200120,China [2]Institute of Gallstone Disease,School of Medicine,Tongji University,Shanghai 200331,China [3]Department of Ultrasonography,Shanghai East Hospital,School of Medicine,Tongji University,Shanghai 200120,China [4]Department of Internal Medicine of Traditional Chinese Medicine,Shanghai East Hospital,School of Medicine,Tongji University,Shanghai 200120,China

出　　处：《World Journal of Gastrointestinal Surgery》2023年第6期1068-1079,共12页世界胃肠外科杂志（英文版）（电子版）

基　　金：Supported by the Pudong New Area Clinical Traditional Chinese Medicine of Top Discipline Project,No.PDZY-2018-0603 and the Featured Clinical Discipline Project of Shanghai Pudong,No.PWYts2021-06.

摘　　要：BACKGROUND Impaired interstitial cells of Cajal(ICCs)are central to the pathophysiology of acute cholecystitis(AC).Common bile duct ligation is a common model of AC,producing acute inflammatory changes and decrease in gallbladder contractility.AIM To investigate the origin of slow wave(SW)in the gallbladder and the effect of ICCs on gallbladder contractions during the process of AC.METHODS Methylene blue(MB)with light was used to establish selective impaired ICCs gallbladder tissue.Gallbladder motility was assessed using the frequency of SW and gallbladder muscle contractility in vitro in normal control(NC),AC12h,AC24h,and AC48h groups of guinea pigs.Hematoxylin and eosin and Massonstained gallbladder tissues were scored for inflammatory changes.ICCs pathological changes alterations were estimated using immunohistochemistry and transmission electron microscopy.The alterations of c-Kit,α-SMA,cholecystokinin A receptor(CCKAR),and connexin 43(CX43)were assessed using Western blot.RESULTS Impaired ICCs muscle strips resulted in the decrease in gallbladder SW frequency and contractility.The frequency of SW and gallbladder contractility were significantly lower in the AC12h group.Compared with the NC group,the density and ultrastructure of ICCs were remarkably impaired in the AC groups,especially in the AC12h group.The protein expression levels of c-Kit were significantly decreased in the AC12h group,while CCKAR and CX43 protein expression levels were significantly decreased in the AC48h group.CONCLUSION Loss ICCs could lead to a decrease in gallbladder SW frequency and contractility.The density and ultrastructure of ICCs were clearly impaired in the early stage of AC,while CCKAR and CX43 were significantly reduced at end stage.

关 键 词：Interstitial cells of Cajal Acute cholecystitis Slow wave GALLBLADDER CONTRACTILITY 

分 类 号：R575.6[医药卫生—消化系统]