Anti-bacterial mechanism of baicalin-tobramycin combination on carbapenem-resistant Pseudomonas aeruginosa  

作　　者：Li-Min Jin Hui Shen Xing-Ying Che Ye Jin Chun-Mei Yuan Neng-Hua Zhang 

机构地区：[1]Laboratory Department,Jiaxing Hospital of Traditional Chinese Medicine,Jiaxing 314001,Zhejiang Province,China

出　　处：《World Journal of Clinical Cases》2023年第17期4026-4034,共9页世界临床病例杂志

基　　金：Supported by Jiaxing Science and Technology Planning Project,No.2019AY32006 and No.2020AY30004。

摘　　要：BACKGROUND Pseudomonas aeruginosa(P.aeruginosa)is an important cause of nosocomial infections,and contributes to high morbidity and mortality,especially in intensive care units.P.aeruginosa is considered a'critical'category bacterial pathogen by the World Health Organization to encourage an urgent need for research and development of new antibiotics against its infections.AIM To investigate the effectiveness of baicalin combined with tobramycin therapy as a potential treatment method for carbapenem-resistant P.aeruginosa(CRPA)infections.METHODS Polymerase chain reaction(PCR)and RT-PCR were used to detect the expression levels of drug-resistant genes(including VIM,IMP and OprD2)and biofilmrelated genes(including algD,pslA and lasR)in CRPA that confer resistance to tobramycin,baicalin and tobramycin combined with baicalin(0,1/8,1/4,1/2 and 1MIC).RESULTS There was a correlation between biofilm formation and the expression of biofilmrelated genes.In addition,VIM,IMP,OprD2,algD,pslA and lasR that confer biofilm production under different concentrations in CRPA were significantly correlated.The synergistic effect of baicalin combined with tobramycin was a significant down-regulation of VIM,IMP,algD,pslA and lasR.CONCLUSION Baicalin combined with tobramycin therapy can be an effective treatment method for patients with CRPA infection.

关 键 词：BAICALIN Pseudomonas aeruginosa TOBRAMYCIN Carbapenem-resistant Pseudomonas aeruginosa Therapy 

分 类 号：R446.5[医药卫生—诊断学]