CCNB1在肝癌中的表达及沉默CCNB1对肝癌细胞的影响  

Expression of CCNB1 in hepatocellular carcinoma and the effect of silencing CCNB1 on hepatocellular carcinoma cells

作　　者：王雪轩 张书铭 杨英 张义炜 潘尚领 彭均华 Wang Xuexuan;Zhang Shuming;Yang Ying;Zhang Yiwei;Pan Shangling;Peng Junhua(Department of Pathophysiology,School of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学基础医学院病理生理学教研室,南宁530021

出　　处：《广西医科大学学报》2023年第5期792-798,共7页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.32060188)。

摘　　要：目的:探讨细胞周期蛋白B1(CCNB1)在肝癌(HCC)中的表达及沉默CCNB1基因对肝癌细胞的影响。方法:利用Gene Expression Omnibus(GEO)和Genotype-Tissue Expression(GTEx)数据库中的HCC测序数据,对CCNB1 mRNA的表达水平及其与预后的相关性进行综合分析。在Huh7、SK两种HCC细胞系中沉默CCNB1(sh-CCNB1)检测其对HCC细胞生长、迁移、侵袭和细胞周期的影响。结果:测序数据分析发现,与正常组织比较,CCNB1 mRNA表达水平在多种肿瘤组织中显著升高,在HCC组织中也显著升高(SMD=1.69,95%CI:1.36~2.02,I2=96.2%)。在Huh7、SK细胞系中下调CCNB1表达结果发现,相对于对照组(sh-NC组),sh-CCNB1组细胞增殖受到明显抑制,细胞迁移和侵袭能力均显著下降;与sh-NC组相比,sh-CCNB1使Huh7细胞G0/G1期占比增加(P<0.05),S期减少(P<0.05);使SK细胞S期占比减少(P<0.000 1)、G2/M期占比显著增多(P<0.01)。结论:CCNB1在HCC中高表达,且沉默CCNB1抑制肝癌细胞的生长。Objectives:To investigate the expression of cyclin B1(CCNB1)in hepatocellular carcinoma(HCC)and the effect of silencing CCNB1 on hepatocellular carcinoma cells.Methods:HCC sequencing data from the databases Gene Expression Omnibus(GEO)and the Genotype-Tissue Expression(GTEx)were used to comprehensively analyze the expression level of CCNB1 mRNA and its correlation with clinical prognosis.CCNB1(sh-CCNB1)was silenced in Huh7 and SK HCC cell lines to detect its effect on the growth,migration,invasion and cell cycle of HCC cells.Results:Analysis of sequencing data showed that CCNB1 mRNA was significantly elevated in various tumor tissues and also in HCC tissues compared with normal tissues(SMD=1.69,95%CI:1.36-2.02,I²=96.2%).The down-regulation of CCNB1 expression in Huh7 and SK cell lines showed that compared with the control group(sh-NC group),the cell proliferation of sh-CCNB1 group was significantly inhibited,and the abilities of cell migration and invasion were significantly decreased;compared with the sh-NC group,sh-CCNB1 increased the proportion of Huh7 cells in G0/G1 phase(P<0.05)and decreased in S phase(P<0.05);sh-CCNB1 decreased the proportion of SK cells in S phase(P<0.0001)and significantly increased the proportion in G2/M phase(P<0.01).Conclusion:CCNB1 is highly expressed in HCC and the silencing of CCNB1 inhibits the growth of HCC cells.

关 键 词：肝细胞癌 细胞周期蛋白B1 基因表达 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]