ABCC5在肺鳞状细胞癌中的表达及临床意义  

Expression and clinical significance of ABCC5 in lung squamous cell carcinoma

作　　者：王君萍 唐晓峰 蒙桂容 李祖云[1] Wang Junping;Tang Xiaofeng;Meng Guirong;Li Zuyun(Department of Pathology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院病理科,南宁530021

出　　处：《广西医科大学学报》2023年第5期877-883,共7页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81760420)。

摘　　要：目的:探讨ATP结合盒亚家族C成员5(ABCC5)在肺鳞状细胞癌(LUSC)中的表达及临床意义。方法:通过癌症基因组图谱(TCGA)、基因表达综合数据库(GEO)、ArrayExpress数据库获取相关数据集,整合分析ABCC5 mRNA在LUSC组织、非LUSC组织中的表达水平,并绘制综合受试者工作特征(sROC)曲线。采用实时荧光定量聚合酶链式反应(RT-qPCR)检测在LUSC细胞系(NCI-H1703、NCI-H520)和人正常肺上皮细胞(BEAS-2B)中ABCC5 mRNA的表达,免疫组织化学染色法(IHC)检测61例LUSC组织及47例对应癌旁组织中ABCC5蛋白表达,同时收集LUSC患者的临床病理信息并进行随访,分析ABCC5的表达与肺鳞癌患者临床病理特征及预后的关系。结果:基于公共数据库,荟萃分析结果提示ABCC5 mRNA在LUSC组织中的表达水平显著上调(SMD=1.56,95%CI:1.23~1.89,I2=78.6%,P<0.001),sROC曲线下面积(AUC)为0.95(95%CI:0.93~0.97)。与BEAS-2B相比,ABCC5 mRNA在NCI-H1703、NCI-H520中表达上调(P<0.05)。IHC结果提示,ABCC5蛋白在LUSC组织中的表达高于癌旁组织(P<0.05),且在T分期、淋巴结转移和临床分期中的比较,差异具有统计学意义(P<0.05)。生存分析显示LUSC患者中ABCC5高表达组与低表达组总生存率和无进展生存率差异无统计学意义(P>0.05)。结论:ABCC5在LUSC中呈高表达,可能与LUSC的形成与进展相关。Objective:To investigate the expression and clinical significance of ATP-binding cassette subfamily C member 5(ABCC5)in lung squamous cell carcinoma(LUSC).Methods:The relevant data sets from the Cancer Genome Atlas(TCGA),Gene Expression Omnibus(GEO)and ArrayExpress databases were integrated and analyzed to explore the expression ofABCC5 mRNA in LUSC and non-LUSC tissues,and the summary receiver operating characteristic(sROC)curve was drawn.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression ofABCC5 mRNA in LUSC cell lines(NCI-H1703,NCI-H520)and human normal lung epithelial cells(BEAS-2B).Immunohistochemical(IHC)staining was used to detect the expression of ABCC5 protein in 61 cases of LUSC tissues and 49 cases of corresponding adjacent tissues.The clinicopathological information of LUSC patients was collected and followed up to analyze the relationship between ABCC5 expression and clinicopathological features and prognosis of patients with lung squamous cell carcinoma.Results:Based on public databases,meta-analysis results suggested that the expression level of ABCC5 mRNA was significantly up-regulated in LUSC tissues(SMD=1.56,95%CI:1.23-1.89,I2=78.6%,P<0.001),and the area under the sROC curve(AUC)was 0.95(95%CI:0.93-0.97).Compared with BEAS-2B cells,ABCC5 mRNA expression was up-regulated in NCI-H1703 and NCI-H520 cells(P<0.05).IHC results showed that the expression of ABCC5 protein in LUSC tissues was higher than that in adjacent tissues(P<0.05),and the differences in the expression of ABCC5 protein in T stage,lymph node metastasis and clinical stage were statistically significant(P<0.05).Survival analysis showed that there was no significant difference in overall survival rate and progression-free survival rate between the high ABCC5 expression group and the low ABCC5 expression group in LUSC patients(P>0.05).Conclusion:ABCC5 is highly expressed in LUSC,which may be related to the formation and progression of LUSC.

关 键 词：肺鳞状细胞癌 ATP结合盒亚家族C成员5 

分 类 号：R734.2[医药卫生—肿瘤]