基于GEO数据库永久性房颤的生物信息学分析及潜在有效药物预测  

BIOINFORMATIC ANALYSIS OF PERMANENT ATRIAL FIBRILLATION BASED ON GEO DATABASE AND THE POTENTIALLY EFFECTIVE DRUGS

作　　者：周斌 王艳 赵明明 刘洪云 王英芝 周纪星 ZHOU Bin;WANG Yan;ZHAO Ming-ming(Linyi City Central Hospital,Linyi,China 276400)

机构地区：[1]临沂市中心医院,山东沂水276400

出　　处：《山东医学高等专科学校学报》2023年第4期262-265,F0003,共5页Journal of Shandong Medical College

摘　　要：目的探讨PAF的治疗靶点及药物,为其临床诊疗提供新的理论和方法。方法利用GSE2240芯片,选择GPL96基因注释平台采集10例PAF患者和20例窦性心律对照者右心耳芯片数据。应用limma软件包筛选DEGs;应用clusterProfiler软件包进行GO、KEGG富集分析;应用STRING11.0在线数据库进行PPI分析,筛选关键基因;应用CMAP数据库筛选与DEGs负相关的小分子药物。结果PAF组共筛选出184个DEGs。GO:DEGs参与多种信号通路等生物过程。KEGG:DEGs主要富集于PI3K-Akt信号通路、松弛素信号通路、AGE-RAGE信号通路、细胞外基质与其受体的相互作用、蛋白质的消化与吸收、HIF-1信号通路等。PPI:10个关键基因,其中7个表达上调,3个表达下调。成功筛选到11种与永久性房颤DEGs负相关的小分子药物,其中布美他尼、石蒜碱最有潜力成为治疗PAF的有效药物。结论NKCC1是PAF潜在的治疗靶点;布美他尼,石蒜碱是潜在治疗PAF的有效药物。Objective To explore the therapeutic targets and drugs for permanent atrial fibrillation(PAF)and to provide new theories and methods for clinical diagnosis and treatment.Methods GSE2240 chip was used and the GPL96 gene annotation platform was chosen to collect the chip data of right atrial appendage in 10 patients with PAF and 20 controls with sinus rhythm.Limma software package was used to screen DEGs.Cluster Profiler software package was used for GO and KEGG enrichment analysis.PPI analysis was performed by using STRING11.0 online database to screen the key genes.CMAP database was used to screen small-molecular drugs which are negatively related to DEGs.Results 184 DEGs were screened out from the PAF group.The GO enrichment analysis showed that DEGs participated in multiple signal pathways.The KEGG enrichment analysis showed that DEGs were mainly concentrated in the PI3K-Akt signal pathway,the relaxin signal pathway,the AGE-RAGE signal pathway,the interaction be-tween extracellular matrix and its receptor,the protein digestion and absorption and the HIF-1 signal pathway,etc.The PPI analysis showed that in the 10 key genes,7 are up-regulated and 3 are down-regu-lated.Eleven small-molecular drugs which are negatively related to DEGs of OAF were successfully screened out,of which bumetanil and lycorine are the most potentially effective drugs for the treatment of PAF.Conclusion NKCC1 is a potential therapeutic target for PAF.Bumetanil and lycorine are the poten-tially effective drugs for the treatment of PAF.

关 键 词：永久性房颤 基因表达 计算生物学 

分 类 号：R473[医药卫生—护理学]