电针百会、神庭对大脑中动脉栓塞大鼠学习记忆功能和前额叶皮层GABAA受体及PSD-95表达的影响  

Effects of electroacupuncture on Baihui and Shenting on learning and memory function and expression of GABAA receptor and PSD-95 in the prefrontal cortex of MCAO rats model

作　　者：夏金言 李钻芳[2,3] 杨嘉誉 林如辉[2] XIA Jinyan;LI Zuan-fang;YANG Jiayu(Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fujian,Fuzhou,350122)

机构地区：[1]福建中医药大学中西医结合研究院,福建省福州市350122 [2]福建中医药大学科技创新与转化中心 [3]福建中医药大学康复医疗技术国家地方联合工程研究中心

出　　处：《中国康复医学杂志》2023年第6期730-736,共7页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金项目(81774386,81803883);福建省自然科学基金项目(2019J01356)。

摘　　要：目的:观察电针百会、神庭对MCAO大鼠学习记忆功能及前额叶皮层γ-氨基丁酸A型(gamma-aminobutyric acid,GABAA)受体及突触后密度蛋白-95(postsynaptic density protein-95,PSD-95)表达的影响,探讨电针对MCAO大鼠学习记忆功能的影响及作用机制。方法:改良Longa线栓阻塞法制备左侧大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)模型,将造模成功的雄性Sprague-Dawley大鼠随机分成模型组、电针组(百会、神庭)和电针非穴组,同时另设假手术组,每组10只。电针组和非穴组进行连续7d电针干预,假手术组和模型组不做任何处理。采用小动物磁共振成像仪检测干预前后大鼠脑梗死体积变化;Morris水迷宫评价大鼠学习记忆功能;HE染色观察缺血侧前额叶皮层病理变化;Western Blot法检测缺血侧前额叶皮层GABAA受体及PSD-95蛋白表达水平。结果:干预前各组大鼠脑梗死体积无显著性差异(P>0.05),随着干预时间延长,与模型组和非穴组相比,电针组行为学评分明显降低(P<0.05);电针组Morris水迷宫学习记忆测试逃避潜伏期缩短(P<0.05)。干预后,与模型组和非穴组相比,电针组脑梗死体积明显减小(P<0.01);电针组神经元密度和神经细胞数量明显增加;电针组前额叶皮层GABAA受体及PSD-95蛋白表达均明显上升(P<0.05),而模型组和非穴组之间无明显差异(P>0.05)。结论:电针百会、神庭能有效减轻脑缺血后神经损伤,改善学习记忆功能,可能与增加其前额叶皮层神经元数量,促进GABAA受体及PSD-95表达有关。Objective:To observe the effect of electroacupuncture on Baihui and Shenting on learning and memory function and the expression of gamma-aminobutyric acid(GABAA)receptor and postsynaptic density protein-95(PSD-95)in the prefrontal cortex of middle cerebral artery occlusion(MCAO)rats model,and to explore the effect and mechanism of electroacupuncture on learning and memory function of MCAO rats.Method:The left middle cerebral artery occlusion model was established with the modified Longa methods.Male Sprague-Dawley rats with successful modeling were randomly divided into four groups:the model group,the electroacupuncture group(Baihui,Shenting),the electroacupuncture non-acupoint group,and the sham operation group,with 10 rats in each group.The electroacupuncture group and the non-acupoint group received electroacupuncture intervention for 7 consecutive days,and the sham operation group and the model group did not receive any treatment. MRI was used to detect the changes of cerebral infarction volume before and afterintervention;the Morris water maze was used to evaluate the learning and memory function of rats;HE stainingwas used to observe the pathological changes of the ischemic prefrontal cortex;and Western Blot wasused to detect the ischemic prefrontal cortex γ-aminobutane expression levels of GABAA receptors and PSD-95proteins.Result:Before the intervention, there was no significant difference in the volume of cerebral infarction betweenthe groups (P>0.05). Compared with the model group and the non-acupoint group,the behavioral scoreof the electroacupuncture group was significantly lower with the prolongation of the intervention time(P<0.05).In the electroacupuncture group, the escape latency of the Morris water maze learning and memory test wasshortened (P<0.05). After intervention,the volume of cerebral infarction in the electroacupuncture group wassignificantly reduced compared with the model group and the non-acupoint group(P<0.01);the density of neuronsand the number of nerve cells in the electroacupunc

关 键 词：γ-氨基丁酸A型受体 突触后密度蛋白-95 电针 脑缺血损伤 学习记忆功能障碍 

分 类 号：R741[医药卫生—神经病学与精神病学] R493[医药卫生—临床医学]