姜黄素对大鼠脑缺血再灌注损伤的治疗作用及其机制  

Therapeutic effect and mechanism of curcumin on cerebral ischemia-reperfusion injury rats

作　　者：杨楚瑜 魏建明 曹湘玉 郭程程 黄雅蓓 程子豪 YANG Chuyu;WEI Jianming;CAO Xiangyu;GUO Chengcheng;HUANG Yabei;CHENG Zihao(Medical School,Hunan University of Medicine,Huaihua 418000,China)

机构地区：[1]湖南医药学院医学院,湖南怀化418000

出　　处：《山东医药》2023年第20期16-19,共4页Shandong Medical Journal

基　　金：湖南省大学生创新创业训练计划项目(S202112214018,S2022122140014);湖南省中医药科研计划项目(2021219);湖南省教育厅科学研究项目(21C1376)。

摘　　要：目的 观察姜黄素对大鼠脑缺血再灌注损伤的治疗作用,并探讨其机制。方法 SD大鼠60只,随机分成姜黄素组、模型组、假手术组,每组20只。姜黄素组和模型组采用线栓法制备大鼠大脑中动脉闭塞模型,造模成功后分别腹腔注射姜黄素(200 mg/kg)和等量生理盐水,连续6 d;假手术组仅剥离颈总动脉、颈外动脉,不结扎动脉,不插线栓。采用Bederson评分评价大鼠神经功能缺损程度,TTC染色观察并计算脑组织梗死体积百分比,Western blotting法检测脑组织中的过氧化物酶体增殖物激活受体γ(PPAR-γ)、凋亡相关斑点样蛋白(ASC)、半胱氨酸天冬氨酸蛋白水解酶1(Caspase-1)蛋白,ELISA法检测血浆肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)。结果 造模后1、7 d模型组、姜黄素组神经功能缺损程度评分高于假手术组(P均<0.05);造模后7 d姜黄素组神经功能缺损程度评分低于模型组(P<0.05)。模型组脑梗死体积百分比高于假手术组,姜黄素组脑梗死体积百分比低于模型组(P均<0.05)。模型组脑组织PPAR-γ蛋白表达低于假手术组,ASC、Caspase-1蛋白表达及血浆TNF-α、IL-1β水平高于假手术组(P均<0.05);姜黄素组脑组织PPAR-γ蛋白表达高于模型组,ASC、Caspase-1蛋白表达及血浆TNF-α、IL-1β水平低于模型组(P均<0.05)。结论 姜黄素可保护脑缺血再灌注损伤大鼠的神经功能,减小脑梗死体积,其机制可能与调节PPAR-γ、ASC、Caspase-1表达及下调TNF-α、IL-1β水平有关。Objective To observe the therapeutic effect of curcumin on cerebral ischemia-reperfusion injury rats and to explore its mechanism.Methods Sixty Sprague-Dewley(SD) rats were randomly divided into the curcumin group,model group,and sham group,with 20 rats in each group.Rats in the curcumin group and model group were treated with thread occlusion method to make middle cerebral artery occlusion(MCAO) models in rats.After successful modeling,curcumin(200 mg/kg) and the same amount of saline solution were injected intraperitoneally,respectively,for 6 consecutive days.In the sham group,only the common carotid artery and external carotid artery were stripped,and no arteries were ligation and no thread plugs were inserted.Bederson score was used to evaluate the severity of neurological impairment in rats,and TTC staining was used to observe and calculate the percentage of infarct volume in the brain tissues.Peroxisome proliferator-activated receptor γ(PPAR-γ),apoptosis-related blotch-like protein(ASC),and Caspase-1 protein in the brain tissues were detected by Western blotting.Plasma tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were detected by ELISA.Results On the 1st and 7th days after modeling,the neurological deficit scores in the model group and curcumin group were higher than those in the sham group(P<0.05).On the 7th day after modeling,the neurological deficit score of the curcumin group was lower than that of the model group(P<0.05).The percentage of cerebral infarction volume in the model group was higher than that in the sham group,and the percentage of cerebral infarction volume in the curcumin group was lower than that in the model group(all P<0.05).The expression of PPAR-γ protein in the brain tissues of the model group was lower than that of the sham group,and the expression of ASC,Caspase-1 protein and plasma levels of TNF-α and IL-1β were higher than those of the sham group(all P<0.05).The expression of PPAR-γ protein in the brain tissues of the curcumin group was higher than that of

关 键 词：姜黄素 脑缺血再灌注损伤 PPAR-γ蛋白 ASC蛋白 Caspase-1蛋白 

分 类 号：R743.3[医药卫生—神经病学与精神病学]