血清外泌体MT1-MMP mRNA在胃癌患者中的表达及其与预后的关系  

Expression of serum exosome MT1-MMP mRNA and its relationship with prognosis in patients with gastric cancer

作　　者：林耀庭 王艳良 武晓勇 张振胜 谭义炫 LIN Yaoting;WANG Yanliang;WU Xiaoyong;ZHANG Zhensheng;TAN Yixuan(Department of General Surgery,Danzhou People's Hospital,Hainan Medical College,Danzhou 571700,Hainan,China)

机构地区：[1]海南医学院附属儋州市人民医院普通外科,海南儋州571700

出　　处：《西部医学》2023年第7期996-1000,共5页Medical Journal of West China

基　　金：海南省卫生计生行业科研课题(19A200054)。

摘　　要：目的 探讨血清外泌体膜1型基质金属蛋白酶(MT1-MMP)mRNA在胃癌患者中的表达及其与预后的关系。方法 选取2016年3月—2018年7月我院行胃癌切除术的98例患者为研究对象。采用实时荧光定量PCR(RT-PCR)测定患者癌组织、癌旁正常组织中MT1-MMP mRNA表达量,分析患者临床病理参数与胃癌组织中MT1-MMP mRNA表达量的关系;对98例患者进行随访,统计术后3年内存活率,比较存活亚组(n=62)与死亡亚组(n=36)患者入组时的胃癌组织与癌旁组织中MT1-MMP mRNA表达水平,运用受试者工作曲线(ROC)分析MT1-MMP mRNA表达水平对术后3年内生存状态的预测效能。结果 98例患者胃癌组织中MT1-MMP mRNA表达水平(0.96±0.12)显著高于癌旁组织(0.80±0.09)(P<0.05);患者胃癌组织中MT1-MMP mRNA表达量与淋巴结转移、浸润程度、TNM分期有关(P<0.05),与性别、年龄、肿瘤位置、肿瘤直径大小及分化程度无关(P>0.05);98例患者术后3年存活亚组入组时的胃癌组织及癌旁组织中MT1-MMP mRNA表达水平显著高于死亡亚组(P<0.05);ROC曲线分析显示,胃癌组织、癌旁组织MT1-MMP mRNA表达水平对患者术后3年生存状态的预测效能有一定局限性(AUC=0.760、0.691),胃癌组织与癌旁组织联合检验的预测效能明显高于单一检测(AUC=0.813)。结论 胃癌组织中MT1-MMP mRNA表达与肿瘤的淋巴转移、TNM分期及浸润程度相关,可一定程度上指导预后。Objective To analyze the expression of serum exosome membrane type 1-matrix metalloproteinase(MT1-MMP)mRNA in patients with gastric cancer and its relationship with prognosis.Methods 98 patients who underwent gastric cancer resection in the hospital from March 2016 to July 2018 were selected as the research subjects.RT-PCR was used to determine the expressions of MT1-MMP mRNA in cancer tissues and adjacent normal tissues of patients.The relationship between clinicopathological parameters and expression level of MT1-MMP mRNA in gastric cancer tissues was analyzed.Follow-up was performed on 98 patients,and the survival rate within 3 years after surgery was statistically analyzed.The expression levels of MT1-MMP mRNA in gastric cancer tissues and adjacent tissues of patients in survival subgroup and death subgroup were compared,and receiver operating characteristic curve(ROC)was used to analyze the predictive efficiency of MT1-MMP mRNA expression level on survival status within 3 years after surgery.Results The expression level of MT1-MMP mRNA with(0.96±0.12)in gastric cancer tissues of 98 patients was obviously higher than(0.80±0.09)in adjacent tissues(P<0.05).The expression level of MT1-MMP mRNA in gastric cancer tissues of patients was related to the lymph node metastasis,degree of infiltration,and TNM staging(P<0.05),but was not related to gender,age,tumor location,tumor diameter and differentiation degree(P>0.05).Among the 98 patients,the expression levels of MT1-MMP mRNA in gastric cancer tissues and adjacent tissues of survival subgroup(n=62)at 3 years after surgery were markedly higher compared with those in death subgroup(n=36)(P<0.05).ROC curve analysis showed that the expression level of MT1-MMP mRNA in adjacent tissues had obvious limitations in predicting the survival status at 3 years after surgery(AUC=0.691),and the combined detection of gastric cancer tissues and adjacent tissues had good predictive efficiency(AUC=0.813).Conclusion The expression of MT1-MMP mRNA in gastric cancer tissues is related

关 键 词：胃癌 实时荧光定量PCR 膜1型基质金属蛋白酶 ROC曲线 

分 类 号：R735.2[医药卫生—肿瘤]