lncRNA H19和SIRT6在人胃癌组织中的表达及其与临床病理的相关性  

Expressions of lncRNA H19 and SIRT6 in human gastric cancer tissues and their correlation with clinicopathology

作　　者：寇耀[1] 贾映东[1] 杨宁波[1] 杨学军[1] 张刘平[1] KOU Yao;JIA Yingdong;YANG Ningbo;YANG Xuejun;ZHANG Liuping(Department of Gastrointestinal Surgery,Suining Central Hospital,Suining 629000,Sichuan,China)

机构地区：[1]遂宁市中心医院胃肠外科,四川遂宁629000

出　　处：《西部医学》2023年第7期1006-1010,共5页Medical Journal of West China

基　　金：四川省中医药管理局科学技术研究专项课题(2021MS258)。

摘　　要：目的 探讨长链非编码RNA(lncRNA)H19和沉默信息调节因子6(SIRT6)在人胃癌组织中的表达及其与临床病理的相关性。方法 选取2018年1月—2021年1月在本院接受手术治疗的96例胃癌患者作为研究对象,收集患者胃癌组织和癌旁正常组织(距离胃癌组织>5 cm)标本,比较人胃癌组织和癌旁正常组织中lncRNA H19、SIRT6表达情况,并分析lncRNA H19、SIRT6表达与胃癌患者临床病理特征的关系。结果 人胃癌组织中lncRNA H19 mRNA表达水平显著高于癌旁正常组织(P<0.05);人胃癌组织中SIRT6 mRNA表达水平和SIRT6蛋白阳性表达率显著低于癌旁正常组织(P<0.05);lncRNA H19 mRNA表达水平与胃癌患者分化程度、浸润深度、肿瘤TNM分期以及淋巴结转移呈正相关(P<0.05);SIRT6 mRNA和SIRT6蛋白表达与胃癌患者分化程度、浸润深度、肿瘤TNM分期以及淋巴结转移呈负相关(P<0.05)。结论 lncRNA H19在人胃癌组织中上调表达,SIRT6在人胃癌组织中下调表达,lncRNA H19、SIRT6表达与胃癌患者临床病理特征存在一定的相关性。Objective To analyze the expressions of long non-coding RNA(lncRNA)H19 and silent information regulator 6(SIRT6)in human gastric cancer tissues and their correlation with clinicopathology.Methods 96 patients with gastric cancer were selected as the research subjects,and the specimens of gastric cancer tissues and adjacent normal tissues(>5 cm from the gastric cancer tissues)were collected.The expressions of lncRNA H19 and SIRT6 in human gastric cancer tissues and adjacent normal tissues were compared,and the relationship between the expressions of lncRNA H19 and SIRT6 and clinicopathological characteristics of patients with gastric cancer was analyzed.Results The expression level of lncRNA H19 mRNA in human gastric cancer tissues was significantly higher than that in adjacent normal tissues(P<0.05).The expression level of SIRT6 mRNA and positive expression rate of SIRT6 protein in human gastric cancer tissues were significantly lower than those in adjacent normal tissues(P<0.05).The expression level of lncRNA H19 mRNA was positively correlated with the degree of differentiation,depth of invasion,tumor TNM stage and lymph node metastasis in patients with gastric cancer(P<0.05).The expressions of SIRT6 mRNA and SIRT6 protein in patients with gastric cancer were significantly negatively correlated with the differentiation degree,invasion depth,tumor TNM stage and lymph node metastasis(P<0.05).Conclusion The expression of lncRNA H19 is up-regulated in human gastric cancer tissues while the expression of SIRT6 is down-regulated.There is a certain correlation between the expressions of lncRNA H19 and SIRT6 and the clinicopathological characteristics of patients with gastric cancer.

关 键 词：长链非编码RNA H19 沉默信息调节因子6 胃癌 临床病理特征 

分 类 号：R735.2[医药卫生—肿瘤] R446[医药卫生—临床医学]