中性蛋白酶水解排水管网沉积物  

Hydrolysis of drainage network sediment by neutral protease

作　　者：邱文韬 张彦平 袁春波[2] 李一兵 冯晓娟 白珺 QIU Wentao;ZHANG Yanping;YUAN Chunbo;LI Yibing;FENG Xiaojuan;BAI Jun(School of Civil Engineering and Transportation,Hebei University of Technology,Tianjin 300401,China;Tianjin Water Conservancy Research Institute,Tianjin 300061,China)

机构地区：[1]河北工业大学土木与交通学院,天津300401 [2]天津市水利科学研究院,天津300061

出　　处：《环境工程学报》2023年第5期1695-1704,共10页Chinese Journal of Environmental Engineering

基　　金：天津市水利科学研究院横向课题(HE2165)。

摘　　要：采用中性蛋白酶水解排水管网沉积物以缓解管网淤堵问题,考察了酶投加量、反应时间、pH、温度等对沉积物水解效果的影响,并通过EPS、三维荧光光谱以及SEM等表征分析方法探讨了可能的机理。结果表明,在酶投量为8%(质量百分数)、pH=7、25℃条件下反应3 h,沉积物的水解效果较好,SCOD由原泥的259.03 mg·L^(-1)升至6715.40 mg·L^(-1),多糖从14.21 mg·L^(-1)升至5797.27 mg·L^(-1),氨氮由23.02 mg·L^(-1)升至47.89mg·L^(-1),有机质含量由50.42%降至48.24%,促进了有机质从固相向液相的迁移。经中性蛋白酶水解后,沉积物的结构和EPS被破坏,内部物质溶出,溶出的多糖占主导地位,且EPS含量变化趋势为S-EPS>TB-EPS>LBEPS。水解后上清液中微生物代谢产物与腐殖质物质增多,蛋白类(酪氨酸、色氨酸)和富里酸类物质减少。以上研究结果可为缓解管网沉积物淤堵提供参考。Neutral protease was used to hydrolyze the sediment of drainage pipe network and alleviate the problem of blockage.The effects of enzyme dosage,reaction time,pH and temperature on sediment hydrolysis were investigated,and the mechanism was explored through EPS,3D EEMs and SEM characterization.The results showed that at enzyme dosage of 8%(w/w),pH=7,25℃and 3h reaction,good sediment hydrolysis was achieved.The SCOD increased from 259.03 mg·L^(−1)to 6715.40 mg·L^(−1),polysaccharide increased from 14.21 mg·L^(−1)to 5797.27 mg·L^(−1),NH_(3)-N increased from 23.02 mg·L^(−1)to 47.89 mg·L^(−1),and organic matter content decreased from 50.42%to 48.24%.This results promoted the migration of organic matters from the solid phase to the liquid phase.After hydrolyzed by neutral protease,the structure of sediment and EPS were destroyed,then the internal substances dissolved out,of which the dissolved amount of polysaccharide dominated,and the varying trend of EPS contents were S-EPS>TB-EPS>LB-EPS.After hydrolysis,the microbial metabolites and humic substances in the supernatant increased,and protein(tyrosine,tryptophan)and fulvic acid substances decreased.The results of this study provides a new method for alleviation of sediment blockage of pipe networks.

关 键 词：中性蛋白酶 排水管网 沉积物 水解 

分 类 号：X703.1[环境科学与工程—环境工程]