基于FT-ICR MS的蒸汽爆破预处理强化污泥厌氧消化的有机物分子解析  

Molecular analysis of organic compounds in anaerobic digestion of sludge enhanced by steam explosion pretreatment based on FT-ICR MS

作　　者：郭送军 韦进毅 王晨路 郝智能 曹宏元 鲍振 刘吉宝[2] 魏源送[2] GUO Songjun;WEI Jinyi;WANG Chenlu;HAO Zhineng;CAO Hongyuan;BAO Zhen;LIU Jibao;WEI Yuansong(School of Resources,Environment and Materials Guangxi University,Nanning 530004,China;Laboratory of Water Pollution Control,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;School of Environmental Science and Engineering,Tianjin Polytechnic University,Tianjin 300387,China;State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;Beijing Xinruide biotechnology limited company,Beijing 102308,China)

机构地区：[1]广西大学资源环境与材料学院,南宁530004 [2]中国科学院生态环境研究中心水污染控制实验室,北京100085 [3]天津工业大学环境科学与工程学院,天津300387 [4]中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京100085 [5]北京新锐得生物科技有限公司,北京102308

出　　处：《环境工程学报》2023年第4期1337-1345,共9页Chinese Journal of Environmental Engineering

基　　金：国家重点研发计划项目(2019YFC1906502)。

摘　　要：污泥预处理后有机物的释放和组成变化会直接影响厌氧消化产甲烷效率,傅里叶变换离子回旋共振质谱(FT-ICR MS)可以深入解析溶解性有机物(DOM)分子组成的变化。通过蒸汽爆破技术对污泥进行预处理,考察不同预处理条件对污泥溶解性有机物分子组成及厌氧消化的影响。研究结果表明,蒸汽爆破预处理明显提升了污泥中溶解性有机物的浓度(最高可提升2.69倍),而高压短时间的预处理更为明显地提升了污泥累积甲烷产量。基于三维荧光光谱(3D-EEM),预处理后污泥不同DOM组分的荧光区域积分标准体积占比发生变化,但不同实验组在厌氧消化过程中荧光区域积分标准体积占比差异并不明显。基于FT-ICR MS的分子水平分析发现,蒸汽爆破预处理后污泥DOM中可生物降解的脂肪族/蛋白质类和脂质类有机物相对占比(质谱峰相对强度)分别增加了3.30%和9.29%,难降解的木质素/CRAM类有机物占比下降了10.53%,DOM分子的双键当量(DBEw)、氧含量(O/Cw)和芳香度(AImodw)降低。此外,预处理增多了低O/C (0.1~0.4)的可生物降解组分以及与微生物代谢活性有关的含硫DOM分子数量,这可能是厌氧消化产甲烷提升的原因之一。本研究结果可为识别污泥预处理过程中可生物降解和难降解有机物组分及其分子特征提供参考。The release and composition change of organic matter after sludge pretreatment will directly affect the efficiency of anaerobic digestion and methanogenesis.Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR MS)can deeply analyze the change of molecular composition of dissolved organic matter(DOM).The sludge was pretreated by steam explosion technology,and the effects of different pretreatment conditions on the molecular composition of dissolved organic matter and anaerobic digestion of sludge were investigated.The results showed that steam explosion pretreatment significantly increased the concentration of dissolved organic matter in sludge(2.69 times at most),while high-pressure short-time pretreatment significantly increased the cumulative methane production of sludge.Based on three-dimensional fluorescence spectroscopy(3D-EEM),the proportion of integrated standard volume of fluorescence region of different DOM components of pretreated sludge changed,but the proportion of integrated standard volume of fluorescence region in different experimental groups during anaerobic digestion was not significantly different.The molecular level analysis based on FT-ICR MS showed that the relative proportion of biodegradable Aliphatic/protein and Lipid organics(relative intensity of mass spectrum peak)in sludge DOM after steam explosion pretreatment increased by 3.30%and 9.29% respectively,the proportion of refractory Lignin/CRAM organics decreased by 10.53%,and the double bond equivalent(DBEw),oxygen content(O/Cw)and aromaticity(AImodw)of DOM molecules decreased.In addition,pretreatment increased the biodegradable components with low O/C(0.1~0.4)and the number of sulfur-containing DOM molecules related to microbial metabolic activity,which might be one of the reasons for the increase of methane production in anaerobic digestion.The results of this study can provide a reference for identifying the biodegradable and refractory organic components and their molecular characteristics in the sludge pretreatment pr

关 键 词：蒸汽爆破 污泥 厌氧消化 溶解性有机物 FT-ICR MS 

分 类 号：X705[环境科学与工程—环境工程]