固相萃取-高效液相色谱法测定豆豉制品中苯甲酸钠和山梨酸钾含量  

Determination of Sodium Benzoate and Potassium Sorbate in Soybean Products by Solid Phase Extraction-High Performance Liquid Chromatography

作　　者：单晓静 SHAN Xiaojing(Guangdong Meiweixian Flavoring Foods Co.,Ltd.,Zhongshan 528437,China)

机构地区：[1]广东美味鲜调味食品有限公司,广东中山528437

出　　处：《食品安全导刊》2023年第18期34-36,共3页China Food Safety Magazine

摘　　要：建立了固相萃取-高效液相色谱法测定豆豉制品中苯甲酸钠、山梨酸钾的方法。样品经提取后,经C_(18) SPE固相萃取小柱净化,C18色谱柱进行分离,DAD检测器进行信号检测。结果表明该方法检出限低,目标物浓度在1~50 mg·L^(-1)信号强度和浓度呈良好的线性关系,并具有良好的精密度与准确度,可作为检测豆豉制品中苯甲酸钠、山梨酸钾的有效定量方法。A method was developed for the determination of sodium benzoate and potassium sorbate in tempeh products by solid-phase extraction-high performance liquid chromatography.The extracted samples was purified by C_(18) SPE solid phase extraction column,separated by C_(18) chromatographic column and detected by DAD detector.The method has low detection limits and a good linear relationship when the target in the range of 1 ~ 50 mg·L^(-1),the method also has good precision and accuracy.It can be used as an effective quantitative method to measure sodium benzoate and potassium sorbate in soybean products.

关 键 词：固相萃取 高效液相色谱 豆豉制品 苯甲酸钠 山梨酸钾 

分 类 号：O657.72[理学—分析化学] TS264.2[理学—化学]