流动注射分析仪法检测生活饮用水中氨的方法建立及应用  

Establishment and Application of Flow Injection Analyzer Method for Determination of Ammonia in Drinking Water

作　　者：卢义柱 邓超 盛晓宇 LU Yizhu;DENG Chao;SHENG Xiaoyu(Shucheng County Center for Disease Control and Prevention,Shucheng 231300,China)

机构地区：[1]舒城县疾病预防控制中心,安徽舒城231300

出　　处：《食品安全导刊》2023年第19期99-102,共4页China Food Safety Magazine

摘　　要：目的:建立流动注射分析仪测定生活饮用水中氨的方法,以替代《生活饮用水标准检验方法无机非金属指标》(GB/T 5750.5—2006)中的纳氏试剂分光光度法。方法:样品和试剂通过流动注射分析仪被带入到连续流动的载流液中。基于NH4+离子、水杨酸钠、氯在碱性条件下反应生成显色复合物,以硝普钠(亚基铁氰化钠)为催化剂,在660 nm波长下比色测量。氨标准样品和23份实际水样用纳氏试剂分光光度法检测,与流动注射分析仪法进行对比分析。结果:氨的浓度在0~2.00 mg·L^(-1),流动注射分析仪法的线性相关系数为0.9999;对氨浓度为0.018 mg·L^(-1)的样品进行15次测定,计算得出方法检出限为0.0014 mg·L^(-1);对两种不同浓度的实际水样进行加标回收试验,加标回收率为96.8%~101.7%;对标准样品的实际检测结果在允许误差范围内,精密度优于纳氏试剂分光光度法;经统计分析,流动注射分析仪法标准偏差为0.0039 mg·L^(-1),低于纳氏试剂分光光度法的(0.0244 mg·L^(-1)),t=8.323,P=0.00<0.05,两种检测方法的差异有统计学意义;对23份农村生活饮用水分别用流动注射分析仪和纳氏试剂分光光度法进行测定,比较两种方法的测定结果,t=1.680,P>0.05,两种检测方法结果的差异无显著性。结论:相比于纳氏试剂分光光度法,流动注射分析仪法具有方便、快捷、高效和低消耗等特点,其准确性和自动化程度优于国家标准方法,适用于生活饮用水中的氨指标的检测。Objective:To establish a method for the determination of ammonia in drinking water by flow injection analyzer to replace the spectrophotometric method of Nessler’s reagent in the GB/T 5750.5—2006.Method:The samples and reagents were brought into the continuous flow carrier liquid by the flow injection analyzer.According to the reaction of NH4+,sodium salicylate and chloro-alkaline conditions,color development complex was formed.Sodium nitroprusside(sodium ferricyanide)was used as catalyst,and colorimetric measurements were made at 660 nm wavelength.Ammonia standard samples and 23 actual water samples were detected by spectrophotometry with Nessler’s reagent and compared with flow injection analyzer.Result:When the concentration of ammonia was 0~2.00 mg·L^(-1),the linear correlation coefficient of flow injection analyzer was 0.9999.The sample with ammonia concentration of 0.018 mg·L^(-1) was determined for 15 times,and the detection limit of the method was calculated to be 0.0014 mg·L^(-1).Two kinds of water samples with different concentrations were recovered by adding standard,and the recovery rate was 96.8%~101.7%.The accuracy of the actual test results of the standard samples is better than that of the Nessler’s reagent spectrophotometry within the allowable error range.After statistical analysis,the standard deviation of the flow injection analyzer method was 0.0039 mg·L^(-1),which was lower than 0.0244 mg·L^(-1) of the Nessler’s reagent spectrophotometry method,t=8.323,P=0.00<0.05,and the difference between the two detection methods was statistically significant.23 samples of rural drinking water were determined by flow injection analyzer and Nessler’s reagent spectrophotometry.The results of the two methods were compared,t=1.680,P>0.05,and there was no significant difference between the two methods.Conclusion:Compared with the Nessler’s reagent spectrophotometry,the flow injection analyzer method has the characteristics of convenience,fast,high efficient and low consumption,its accuracy a

关 键 词：流动注射分析仪法 纳氏试剂分光光度法 生活饮用水 氨 

分 类 号：TU991.21[建筑科学—市政工程]