HPLC法测定藏药帕栗嘎不同品种及其代用品中马兜铃酸A的含量  

Determination of Aristolochic Acid A in Different Varieties of Tibetan Medicine Paliga and Its Substitutes by HPLC

作　　者：周则[1] 拉果卓么 次仁白姆 卓玛 张浩[3] ZHOU Ze;LAGUO Zhuome;CIREN Baimu;ZHUO Ma;ZHANG Hao(College of Tibetan Medicine,Qinghai University,Xining 810001,China;Qinghai Provincial Hospital of Tibetan Medicine,Xining 810007,China;West China School of Pharmacy,Sichuan University,Chengdu 610041,China)

机构地区：[1]青海大学藏医学院,青海西宁810001 [2]青海省藏医院,青海西宁810007 [3]四川大学华西药学院,四川成都610041

出　　处：《西部中医药》2023年第7期79-81,共3页Western Journal of Traditional Chinese Medicine

基　　金：国家社科基金西部项目(16XMZ024,16XMZ026);2019年青海省中藏医药(循证)研究课题(2019202)。

摘　　要：目的:测定4种常用藏药帕栗嘎品种(藏马兜铃、Aristolochia sp.、小木通和绣球藤)中马兜铃酸A的含量,明确藏药帕栗嘎不同品种中马兜铃酸A的含量分布情况,为藏药帕栗嘎的临床安全用药提供科学依据。方法:采用高效液相色谱法(high performance liquid chromatography,HPLC),色谱柱:Thermo ODS HYPERSIL(4.6 mm×200 mm,5μm);流动相:甲醇-1.0%甲酸水溶液(70∶30);流速:1.0 mL/min;检测波长:315 nm;柱温:25℃。结果:马兜铃酸A在20~180μg有良好的线性关系;平均加样回收率为95.89%,RSD为1.26%;藏药帕栗嘎4个品种中藏马兜铃中马兜铃酸A的含量为3.5818 mg/g,Aristolochia sp.、小木通和绣球藤中未检测出马兜铃酸A。Aristolochia sp.尚无统一命名和草本考证,为本文首次提出。结论:藏马兜铃中马兜铃酸A含量较高,临床应慎用,应由功效相同而不含马兜铃酸A的“Aristolochia sp.”和毛茛科植物川木通代替。Objective:To provide scientific reference for safe medication of Tibetan medicine Paliga in clinic by determining the contents of aristolochic acid A contained in four kinds of commonly-used Tibetan medicine Paliga varieties,and confirm the distribution of the contents of aristolochic acid A in different varieties of Tibetan medicine Paliga.Methods:HPLC was adopted,chromatographic column:Thermo ODS HYPERSIL 4.6 mm×200 mm column,5μm;mobile phase:methanol-1.0%methane acid aqueous solution(70:30);current velocity:1.0 mL/min;detection wavelength:315 nm;column temperature:25℃.Results:Aristolochic acid A presents good linear relationship in the range between 20 and 180μg;average recovery rate was 95.89%,RSD was 1.26%;Tibetan medicine Paliga owns different contents of aristolochic acid A in four varieties,among them,the contents of aristolochic acid A was 3.5818 mg/g contained in Tibetan Madouling,and aristolochic acid A hasn't been detected in Aristolochia sp.,Xiaomutong and Xiuqiuteng.Among them,there was no unified name and herbal textual research of aristolochia sp.,raised in the paper for the first time.Conclusion:Due to higher contents of aristolochic acid A contained in Tibetan Madouling,it should be cautiously used in clinic in Tibetan medicine,and should be replaced by"Aristolochia sp."and caraciniaceae plant Chuan Mutong,which show the same effects without aristolochic acid A.

关 键 词：帕栗嘎 藏马兜铃 “Aristolochia sp.” 小木通 绣球藤 马兜铃酸A 高效液相色谱法 

分 类 号：R914.1[医药卫生—药物化学]