高活性碱性果胶酶产生菌的筛选、鉴定及发酵条件优化  

Screening,Identification and Fermentation Optimization of Bacteria Strain Producing Alkaline Pectinase with High Activity

作　　者：刘杰 刘婷 唐梅芳 林元山[4] LIU Jie;LIU Ting;TANG Mei-fang;LIN Yuan-shan(Hanxin Fact-seeking Environmental Technology Corporation,Nanchang 330000,China;School of Environmental and Chemical Engineering,Nanchang Hangkong University,Nanchang 330063,China;Anyuan County Agriculture and Rural Bureau,Jiangxi Ganzhou 342199,China;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]汉鑫求实环境技术有限公司,南昌330000 [2]南昌航空大学环境与化学工程学院,南昌330063 [3]安远县农业农村局,江西赣州342199 [4]湖南农业大学生物科学技术学院,长沙410128

出　　处：《南昌航空大学学报（自然科学版）》2023年第2期63-69,共7页Journal of Nanchang Hangkong University(Natural Sciences)

基　　金：国家自然科学基金青年项目(42007431);湖南省高校创新平台开放基金项目(19k045);南昌航空大学博士启动基金(EA202002153)。

摘　　要：通过平板分离法和刚果红染色法,从腐烂苹果中分离得到1株碱性果胶酶产生菌株Lys-3004。经形态观察和16S rDNA扩增子测序分析,确定该菌株为芽孢杆菌属(Bacillus sp.Lys-3004)。通过单因素及正交试验优化产酶条件,结果表明:菌株Lys-3004在7.0%葡萄糖、1.2%黄豆粕、培养基初始pH 7.0、33℃、180 r/min恒温震荡培养96 h的条件下,发酵产生的果胶酶酶活力达到11352.5 U/mL,较优化之前提高了3.7倍。菌株Lys-3004具有应用和深入研究的潜力。An alkaline pectinase producing strain Lys-3004 was isolated from a decayed apple by combination of plate screening and Congo red staining.Based on morphological characteristics and 16S rDNA amplicon sequencing analysis,strain Lys-3004 was identified as Bacillus sp.The optimum fermentation conditions of pectinase activity were performed by single factor and orthogonal experiment.The results were as follows:glucose 7.0%,soybean meal 1.2%,initial pH of medium 7.0,180 r/min at 33℃,culturing for 96 h.Under the optimum conditions above,the enzyme activity of strain Lys-3004 reached 11352.5U/mL,which was 3.7 folds higher than that obtained before optimization.Strain Lys-3004 is capable of potential applications for following researches.

关 键 词：碱性果胶酶 筛选 发酵条件优化 

分 类 号：Q93-33[生物学—微生物学]