沉默SF3B1促进人肺癌细胞系A549凋亡并抑制其增殖及侵袭  

Silencing SF3B1 promotes apoptosis and inhibits proliferation and invasion of human lung cancer cell line A549

作　　者：张小婉 康霞 姚小英 谢芳 李莹 ZHANG Xiaowan;KANG Xia;YAO Xiaoying;XIE Fang;LI Ying(Department of Geriatrics,the General Hospital of Western Theater Command PLA,Chengdu 610083;Department of Oncology,Jintang First People's Hospital,Chengdu 610400;Department of Ultrasound,the General Hospital of Western Theater Command PLA,Chengdu 610083)

机构地区：[1]中国人民解放军西部战区总医院老年医学科,四川成都610083 [2]金堂县第一人民医院肿瘤科,四川成都610400 [3]中国人民解放军西部战区总医院超声科,四川成都610083

出　　处：《基础医学与临床》2023年第8期1265-1270,共6页Basic and Clinical Medicine

摘　　要：目的探索剪接因子3B亚基(SF3B1)对人肺癌细胞凋亡、增殖及侵袭的影响。方法选择西部战区总医院2017年6月~2020年6月确诊的非小细胞肺癌(NSCLC)患者为研究对象,检测癌组织、癌旁组织SF3B1水平,并分析SF3B1与患者临床病理特征、生存预后的关系。培养A549细胞,并将细胞分为对照组、转染si-NC组和si-SF3B1组,流式细胞术测定细胞凋亡,CCK-8法检测细胞的增殖,Transwell实验检测细胞侵袭能力,Western blot检测Ras/Raf/ERK信号蛋白表达。结果NSCLC癌组织中SF3B1 mRNA水平显著高于癌旁组织(P<0.05),A549细胞中SF3B1 mRNA水平显著高于人正常肺上皮细胞(P<0.05);SF3B1与NSCLC患者淋巴结转移、肿瘤大小及分化程度存在相关性(P<0.05);SF3B1高表达患者中位总生存期(OS)、无进展生存期(PFS)均明显低于SF3B1低表达患者(P<0.05);沉默SF3B1后,促进A549细胞凋亡,抑制其增殖、侵袭(P<0.05);沉默SF3B1显著降低A549细胞Ras、Raf及p-ERK蛋白水平(P<0.05)。结论SF3B1在NSCLC高表达,沉默SF3B1可促进肺癌细胞凋亡,抑制细胞增殖、侵袭。Objective To explore the effect of splicing factor 3B subunit(SF3B1)on apoptosis,proliferation and invasion of human lung cancer cells.Methods Non-small cell lung cancer(NSCLC)patients in the General Hospital of Western Theater Command PLA from June 2017 to June 2020 were selected as the research objects to detect the level of SF3B1 in cancer and adjacent tissues,and to analyze the relationship between SF3B1 and the pathological characteristics,survival and prognosis of patients.In addition,A549 cells were cultured and divided into control group,transfected si-NC group and si-SF3B1 group.Cell apoptosis was detected by flow cytometry,cell proliferation was detected by CCK-8,cell invasion was detected by Transwell,and the expression of Ras/Raf/ERK signal protein was detected by Western blot.Results The level of SF3B1 mRNA in cancer tissues was significantly higher than that in adjacent tissues(P<0.05),and its level in A549 cells was significantly higher than that in normal lung epithelial cell line BEAS-2B(P<0.05).The high level of SF3B1 was related to lymph node metastasis,tumor size and differentiation(P<0.05).The median overall survival(OS)and progression free survival(PFS)of patients with low expression of SF3B1 were significantly higher than those with high expression of SF3B1(P<0.05).After silencing SF3B1,the apoptosis of A549 cells was significantly increased but the proliferation and invasion were significantly decreased.The silence of SF3B1 suppressed the expression of Ras,Raf and p-ERK in A549 cells.Conclusions SF3B1 is highly expressed in NSCLC.Silencing SF3B1 can promote lung cancer cell apoptosis,inhibit cell proliferation and invasion.

关 键 词：非小细胞肺癌 剪接因子3B亚基 RAS RAF ERK 

分 类 号：R392.2[医药卫生—免疫学]