蕙兰‘红香妃’内生真菌分离鉴定及体外抑菌效应  

Isolation and identification of endophytic fungi from Cymbidium faberi‘Hongxiangfei’and their bacteriostatic effect in vitro

作　　者：蒋楚楚 辛静静 夏树全 罗平 邵果园[1] 崔永一[1] JIANG Chuchu;XIN Jingjing;XIA Shuquan;LUO Ping;SHAO Guoyuan;CUI Yongyi(College of Horticulture Science,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Tongxing Planting Professional Cooperative of Jiuzhaigou County in Sichuan,Jiuzhaigou 623499,Sichuan,China)

机构地区：[1]浙江农林大学园艺科学学院,浙江杭州311300 [2]四川省九寨沟县同兴种植专业合作社,四川九寨沟623499

出　　处：《浙江农林大学学报》2023年第4期783-791,共9页Journal of Zhejiang A&F University

基　　金：浙江省科学技术厅援助项目(2020C26003)。

摘　　要：【目的】从蕙兰‘红香妃’Cymbidium faberi‘Hongxiangfei’中筛选获得兰科Orchidaceae植物病害生防菌,为蕙兰菌根化栽培及绿色生物防治提供科学依据。【方法】通过蕙兰‘红香妃’健康根段培养、分离、纯化、鉴定内生真菌;以5株常见植物病原菌为指示菌,通过平板对峙培养法筛选出生物防治效果最优的内生真菌,并用活体接种法验证其抑菌效果。【结果】在蕙兰‘红香妃’健康植株根段培养中共分离筛选出6株内生真菌,经鉴定4株属于毛壳属Chaetomium spp.,1株属于镰刀属Fusarium spp.,另1株属于赛多孢属Scedosporium spp.。在平板对峙培养中发现:6株内生真菌对5株病原菌均具有一定的抑菌效果,其中Z2菌株腐皮镰刀菌Fusarium solani对兰花茎腐病致病菌——尖孢镰刀菌F.oxysporum的抑制效果明显,抑制率为67.51%;Z3菌株蝇生二叉毛壳菌Dichotomopilus funicola对兰花炭疽病致病菌——胶孢炭疽菌Colletotrichum gloeosporioides的抑制率达68.56%。活体接种研究表明:经Z3菌株处理,春兰‘绿云’C.goeringii‘Green Cloud’的病叶率、病情指数极显著(P<0.01)低于对照。【结论】Z3菌株是筛选出的防治兰科植物病害的优势拮抗生物防治菌。[Objective]The objective is to screen and obtain the biocontrol fungi against orchid plant diseases from Cymbidium faberi‘Hongxiangfei’,so as to provide scientific basis for mycorrhizal cultivation and green biological control of C.faberi.[Method]The endophytic fungi were isolated,purified and identified from the healthy root segments of C.faberi‘Hongxiangfei’.With 5 common plant pathogens as indicator fungi,the endophytic fungi with the best biocontrol effect were screened by plate confrontation culture method,and their bacteriostatic effect was verified by in vivo inoculation.[Result]6 endophytic fungi were isolated from the root segment culture of healthy plants of C.faberi‘Hongxiangfei’,and 4 strains belonged to Chaetomium spp.,1 strain belonged to Fusarium spp.and 1 belonged to Scedosporium spp.It was found in the plate confrontation culture that all 6 endophytic fungi had certain inhibitory effect on 5 pathogenic strains,among which Z2 strain(Fusarium solani)had an obvious inhibitory effect on F.oxysporum(the causative agent of orchid stem rot),with an inhibition rate of 67.51%.The inhibition rate of Z3 strain(Dichotomopilus funicola)against Colletotrichum gloeosporioides(the pathogen of orchid anthracnose)was 68.56%.The in vivo inoculation test of Cymbidium goeringii‘Green Cloud’showed that the rate of diseased leaves and disease index of the experimental group treated with Z3 strain were significantly lower than those of the control group(P<0.01).[Conclusion]Z3 is selected as the dominant antagonistic biocontrol strains against orchid diseases.

关 键 词：蕙兰‘红香妃’ 内生真菌 毛壳属 镰刀属 抑菌 

分 类 号：S432.4[农业科学—植物病理学]