Heparin/Growth Factors‑Immobilized Aligned Electrospun Nanofibers Promote Nerve Regeneration in Polycaprolactone/Gelatin‑Based Nerve Guidance Conduits  

作　　者：Yasuhiro Ikegami Muhammad Shafiq Shinichi Aishima Hiroyuki Ijima 

机构地区：[1]Department of Chemical Engineering,Faculty of Engineering,Graduate School,Kyushu University,744 Motooka,Nishi‑Ku,Fukuoka 819‑0395,Japan [2]Department of Biotechnology,Faculty of Science and Technology,University of Central Punjab(UCP),Lahore 54000,Pakistan [3]Department of Pathology and Microbiology,Faculty of Medicine,Saga University,Nabeshima 5‑1‑1,Saga 849‑8501,Japan

出　　处：《Advanced Fiber Materials》2023年第2期554-573,共20页先进纤维材料（英文）

基　　金：The part of this research was also funded by Grant-in-Aid for JSPS Fellows(Grant#JP21F21353);JSPS KAKENHI funding(JP18J20984 and JP21H01732).

摘　　要：Injuries to the nervous system account for the widespread morbidity,mortality,and discomfort worldwide.Artificial nerve guidance conduits(NGCs)offer a promising platform for nerve reconstruction,however,they require extracellular matrix(ECM)-like features to better mimic the in vivo microenvironment.Consequently,this research was aimed to fabricate heparin/growth factors(GFs)-immobilized artificial NGCs.Heparin was covalently immobilized onto aligned electrospun polycapro-lactone/gelatin(PCL/Gel)nanofibers.Thereafter,basic fibroblast growth factor(bFGF)and nerve growth factor(NGF)were preferentially immobilized on heparinized nanofibers;the immobilization efficiency of GFs was found to be 50%with respect to(w.r.t.)their initial loaded amounts.The in vivo implantation of NGCs in a sciatic nerve defect model revealed the successful retention(~10%w.r.t the initial loaded amount)and bioactivity of NGF for up to 5 days.The permeability of bovine serum albumin(BSA)from nanofibrous membranes was further assessed and found to be comparable with the commercialized cel-lulose acetate membranes.The bioactivity of NGCs was assessed in a sciatic nerve defect model in rats for short-term(1 week)and long-term(1-month).The NGCs displayed good structural stability and biocompatibility in vivo.The in vivo evaluation revealed the accumulation of host cells into the transplanted NGCs.Taken together;these heparin/GFs-immobilized artificial NGCs may have broad implications for nerve regeneration and related tissue engineering disciplines.

关 键 词：HEPARIN Growth factor-immobilized scaffolds Aligned nanofibers Core/shell nanofibers Nerve tissue engineering Scaffolds Co-axial electrospinning 

分 类 号：TB383[一般工业技术—材料科学与工程]