A network pharmacology-based investigation of the mechanism of action of chrysanthemum in treating dry eye  

作　　者：Li-Yuan Cao Qian Long Peng-Fei Jiang Jun Peng Qing-Hua Peng 

机构地区：[1]College of traditional Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,China [2]Ophthalmology of Traditional Chinese Medicine,the First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China

出　　处：《TMR Pharmacology Research》2023年第2期25-29,共5页TMR药理学研究

基　　金：This study was supported by the following grants:National Natural Science Foundation of China General Program funded projects(30772824,81574031);Hunan Provincial Key Laboratory Construction Project for Traditional Chinese Medicine Prevention and Treatment of Ophthalmic Diseases(2017TP1018);Key Discipline Construction Project of Traditional Chinese Medicine Ophthalmology from State Administration of Traditional Chinese Medicine(ZK 1801YK015);Changsha Science and Technology Plan Projects(K1501014-31,KC1704005);Hunan Provincial Graduate Research and Innovation Projects(CX20220780);Research Innovation Projects for Graduate Students at Hunan University of Chinese Medicine(2022YF01).

摘　　要：Background:Dry eye is a chronic inflammatory disease of the ocular surface that is caused by multiple factors,characterized by pain,visual disturbance,and ocular damage.It is a common ophthalmic disease worldwide and a hot research field for scholars both domestically and internationally.This article employs network pharmacology methods to analyze the mechanism of Chrysanthemum in treating dry eye,which is a promising approach.Methods:The TCMSP(http://tcmspw.com/tcmsp.php)was used to screen for candidate active ingredient molecules of chrysanthemum with oral bioavailability≥30%and drug similarity to chrysanthemum≥0.18 as parameters.The active ingredients of chrysanthemum were searched using the“Related Targets”column in the TCMSP,followed by target prediction.Subsequently,Cytoscape 3.6.0 was employed to construct a compound-target network for chrysanthemum.The Online Mendelian Inheritance in Man and DisGeNET databases were used to identify pathogenic genes associated with dry eye.Furthermore,the STRING database was used to construct an interaction network and bar graph of intersecting target proteins in chrysanthemum to analyze protein interactions and core targets.To obtain key targets of active ingredients of chrysanthemum for treating dry eye,active ingredients targets of chrysanthemum and dry eye targets were intersected using Venny.Finally,a drug-active ingredient-key target-disease network was constructed.Gene Ontology functional annotation of key targets was performed using the WEBGESTALT database,and Kyoto Encyclopedia of Genes and Genomes pathway enrichment was performed using the REACTOM database.Results:Eighty active ingredients of chrysanthemum corresponding to targets were obtained.Among active ingredients,508 predicted targets were identified,along with 4180 genes associated with dry eye and 45 key targets of chrysanthemum for treating dry eye.The functions of key targets primarily include regulation of gene expression,oxidative stress,immune response,apoptosis,proliferation,regulation of ce

关 键 词：CHRYSANTHEMUM dry eye network pharmacology traditional Chinese medicine TARGETS signaling pathway 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]