基于SSR分子标记的多花水仙资源遗传多样性分析  

Genetic Diversity Analysis of Narcissus tazetta Germplasm Resources Based on SSR Marker

作　　者：田奇琳 何炎森 敬月美 余惠文 朱旸[1] 陈郑镔 陆銮眉[1] TIAN Qilin;HE Yansen;JING Yuemei;YU Huiwen;ZHU Yang;CHEN Zhengbin;LU Luanmei(Fujian Provincial University Key Laboratory of Fujian and Taiwan Garden Plants,Minnan Normal University,Zhangzhou Fujian 363000,China;Institute of Subtropical Agriculture,Fujian Academy of Agricultural Sciences,Zhangzhou Fujian 363005,China)

机构地区：[1]闽南师范大学,闽台特色园林植物福建省高校重点实验室,福建漳州363000 [2]福建省农业科学院亚热带农业研究所,福建漳州363005

出　　处：《种子》2023年第5期91-96,F0002,共7页Seed

基　　金：福建省自然科学基金(2021 J 05199,2020 N 0061);漳州市自然科学基金(ZZ 2020 J 35);福建省教育厅中青年教师教育科研项目(JT 180317,JAT 190356);闽南师范大学启动经费(4206-L 21715)。

摘　　要：基于SSR分子标记技术,利用17对引物对21份多花水仙资源进行遗传多样性分析。结果表明,聚丙烯酰胺凝胶电泳共扩增出99条多态性条带,多态位点百分率为93.40%。Nei’s遗传多样性指数均值为0.2667,Shannon信息指数均值为0.4117。主成分分析和UPGMA聚类分析结果显示,供试的21个水仙种源分成四大类,中国水仙单复瓣种质都聚在Ⅰ类群,遗传多样性水平低,洋水仙(Grand Soleil d′Or、Ziva和Pearl)所在的类群(Ⅱ、Ⅲ和Ⅳ)遗传相似系数在0.4~0.8之间,类群间亲缘关系较远。本研究为今后多花水仙传统育种结合生物技术育种提供理论依据。Based on SSR molecular marker technology,17 pairs of primers were used to study the genetic relationship of 21 resources of Narcissus tazetta.The results showed that a total of 99 polymorphic bands were amplified by polyacrylamide gel electrophoresis,and the percentage of polymorphic loci was 93.40%.Nei's genetic diversity average index was 0.2667 and Shannon information average index was 0.4117.The results of principal component analysis and UPGMA clustering showed that 21 Narcissus tazetta resources were divided into four clusters.The single and double petalled type germplasms of Chinese Narcissus comprised Cluster I,within which the genetic diversity was low.The Narcissus tazetta Grand Soleil d′Or,Ziva,and Pearl belonged to ClusterⅡ,ⅢandⅣ,respectively.Similarity coefficients among these three clusters ranged from 0.4 to 0.8,and the genetic distance between each two of them was far.The study proyided a theoretical basis for the future traditional breeding and biotechnology breeding of N.tazetta.

关 键 词：多花水仙 SSR 遗传多样性 亲缘关系 

分 类 号：S682.21[农业科学—观赏园艺]