miR⁃30b⁃5p通过下调MKRN3表达抑制食管癌细胞增殖、迁移和侵袭  

MiR⁃30b⁃5p inhibits the proliferation,migration and invasion of esophageal cancer cells by down⁃regulat⁃ing the expression of MKRN3

作　　者：周乾华 毕华俊 孟凡东 施益明 芮超 ZHOU Qianhua;BI Huajun;MENG Fandong;SHI Yiming;RUI Chao(Department of Thoracic and Cardiovascular Surgery,Anhui Second People′s Hospital,Hefei 233000,China)

机构地区：[1]安徽省第二人民医院胸心外科,合肥230012

出　　处：《实用医学杂志》2023年第13期1627-1633,共7页The Journal of Practical Medicine

基　　金：安徽省卫生健康委科研计划项目(编号:2018SEYL024)。

摘　　要：目的探讨miR⁃30b⁃5p在食管癌细胞中的表达及对食管癌细胞增殖、迁移和侵袭的影响机制。方法收集2016年1月至2020年12月就诊于安徽省第二人民医院手术治疗的70例食管癌患者的肿瘤和癌旁组织,检测miR⁃30b⁃5p的表达水平。qRT⁃PCR检测食管癌细胞株中miR⁃30b⁃5p的表达水平。将miR⁃30b⁃5p mimics转染至TE⁃13和ECA109细胞中,检测转染后各组细胞中miR⁃30b⁃5p及MKRN3表达水平。根据细胞增殖实验,Transwell及裸鼠成瘤实验检测食管癌细胞增殖、迁移及侵袭能力。双荧光素酶实验验证miR⁃30b⁃5p的靶基因。蛋白印迹实验探索miR⁃30b⁃5p对食管癌影响的分子机制。结果miR⁃30b⁃5p在食管癌细胞和食管癌组织中相较于正常上皮细胞和癌旁组织表达显著下降(P<0.05)。细胞增殖实验显示,miR⁃30b⁃5p mimics组增殖率显著低于NC组(P<0.05);Transwell实验结果显示,miR⁃30b⁃5p mimics组迁移率及侵袭率显著低于NC组(P<0.05);双荧光素酶实验显示MKRN3是miR⁃30b⁃5p的直接靶基因。Western blot检测JAK/STAT信号通路蛋白发现,miR⁃30b⁃5p mimics组蛋白低于NC组(P<0.05)。结论miR⁃30b⁃5p在食管癌中呈现低表达,miR⁃30b⁃5p通过下调MKRN3表达抑制食管癌细胞增殖、迁移和侵袭能力。Objective To investigate the expression of miR⁃30b⁃5p in esophageal cancer cells and its effect on the proliferation,migration,and invasion of esophageal cancer cells.Methods The expression level of miR⁃30b⁃5p was detected in tumor and adjacent tissues of 70 patients with esophageal cancer who received surgical treatment in our hospital from January 2016 to December 2020.The expression level of miR⁃30b⁃5p in esophageal cancer cell lines was detected by qRT⁃PCR.miR⁃30b⁃5p mimics were transfected into TE⁃13 and ECA109 cells,and the expression levels of miR⁃30b⁃5p and MKRN3 in the transfected cells were detected.The proliferation,migration and invasion ability of esophageal carcinoma cells were determined by cell proliferation assay,Transwell assay and nude mouse tumorigenesis assay.The target gene of miR⁃30b⁃5p was verified by double luciferase assay.Western blotting was used to explore the molecular mechanism of the effect of miR⁃30b⁃5p on esophageal carcino⁃ma.Results The expression of miR⁃30b⁃5p in esophageal carcinoma cells and tissues was significantly decreased compared with normal epithelial cells and paracancer tissues(P<0.05).Cell proliferation experiment showed that the proliferation rate of miR⁃30b⁃5p mimics group was significantly lower than that of NC group(P<0.05).Tran⁃swell experiment results showed that the mobility and invasion rates of miR⁃30b⁃5p mimics group were significantly lower than those of NC group(P<0.05).Dual luciferase assay showed that MKRN3 was the direct target gene of miR⁃30b⁃5p.Western blot analysis of JAK/STAT signaling pathway protein showed that the histone of miR⁃30b⁃5p mimics was lower than that of NC group(P<0.05).Conclusion miR⁃30b⁃5p shows low expression in esophageal cancer,and miR⁃30b⁃5p inhibits the proliferation,migration and invasion of esophageal cancer cells by down⁃regu⁃lating the expression of MKRN3.

关 键 词：miR⁃30b⁃5p makorin环指蛋白3 食管癌 JAK/STAT信号通路 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R735.1[医药卫生—肿瘤]