TXNDC5 siRNA靶向纳米微粒对胃癌模型动物抑瘤作用的实验研究  

Antitumor effect of TXNDC5 siRNA targeted nanoparticles on animal model of gastric cancer:An experi⁃mental study

作　　者：李相辉 侯艳红 吴凯 杨汨 张林 LI Xianghui;HOU Yanhong;WU Kai;YANG Mi;ZHANG Lin(Department of Gastroenterology,the Eighth Medical Center,PLA General hospital,Beijing 100091,China;Hebei North University,Zhangjiakou 075132,China;不详)

机构地区：[1]中国人民解放军总医院第八医学中心消化内科,北京100091 [2]河北北方学院,河北张家口075132

出　　处：《实用医学杂志》2023年第13期1634-1640,共7页The Journal of Practical Medicine

基　　金：首都卫生发展科研基金项目资助(编号:2011⁃5007⁃01)。

摘　　要：目的探讨装载TXNDC5基因RNA干扰片段(TXNDC5 siRNA)的聚氰基丙烯酸正丁酯纳米微球对人胃腺癌细胞种植瘤荷瘤模型动物的治疗作用。方法采用乳化聚合法制备负载TXNDC5 siRNA的纳米微球(TXNDC5 siRNA⁃PBCA⁃NP)。采用皮下种植法建立胃腺癌荷瘤动物模型,经尾静脉注入TXNDC5 siRNA⁃PBCA⁃NP至荷移植瘤裸鼠体内,同时建立聚氰基丙烯酸正丁酯空纳米微球(PBCA⁃NP)、5氟尿嘧啶(5⁃Fu)及生理盐水空白对照组,治疗后检测在体内情况下各组对种植瘤的抑制情况,进行组间比较。结果TXNDC5 siRNA⁃PBCA⁃NP治疗组小鼠肿瘤抑制率为(66.98±4.02)%,5⁃Fu治疗组小鼠肿瘤抑制率为(64.10±7.55)%,均显著高于其余各对照组(P<0.05),其二者间差异无统计学意义。治疗结束时TXNDC5 siRNA⁃PBCA⁃NP治疗组平均肿瘤质量为(381.27±24.22)mg,5⁃Fu治疗组平均肿瘤质量为(414.53±29.59)mg,均显著低于其他各对照组(P<0.05),其二者间差异无统计学意义。结论初步的胃癌种植瘤裸鼠模型治疗实验显示TXNDC5 siRNA⁃PBCA⁃NP在体内条件下对胃癌治疗作用较为显著,对动物体内的肿瘤具有良好的抑制作用,值得进一步研究。Objective To investigate the therapeutic effect of polybutylcyanoacrylate nanoparticles loaded with TXNDC5 gene RNA interference chip(TXNDC5 siRNA)on human gastric adenocarcinoma cell implanted tumor bearing model animals.Methods Nano⁃microspheres loaded with TXNDC5 siRNA(TXNDC5 siRNA⁃PBCA⁃NP)were prepared by emulsion polymerization.The tumor bearing animal model of gastric adenocarcinoma was established by subcutaneous implantation.TXNDC5 siRNA⁃PBCA⁃NP was injected into nude mice bearing trans⁃planted tumor through caudal vein.At the same time,the blank control group of polybutylcyanoacrylate nanoparticles(PBCA⁃NP),5⁃fluorouracil(5⁃FU)and normal saline groups were established.After treatment,the inhibition of implanted tumor in each group was detected and compared between groups.Results The tumor inhibition rates of the TXNDC5 siRNA⁃PBCA⁃NP treatment group and the 5⁃FU treatment group were(66.98±4.02)%and(64.10±7.55)%,both significantly higher than those of the other control groups(P<0.05).At the end of treatment,the aver⁃age tumor masses of the TXNDC5 siRNA⁃PBCA⁃NP treatment group and 5⁃FU treatment group were(381.27±24.22)mg and(414.53±29.59)mg,both significantly lower than those of other control groups(P<0.05).Conclusion The preliminary therapeutic effect on the nude mouse model of gastric cancer shows that TXNDC5 siRNA⁃PBCA⁃NP has a significant therapeutic effect on tumor in vivo.It could have a promising expectation of further study.

关 键 词：胃癌 纳米微粒 TXNDC5基因 RNA干扰 动物模型 

分 类 号：R735.2[医药卫生—肿瘤]