Effects of Arbuscular Mycorrhizal Fungi on the Physiology and Saponin Synthesis of Paris polyphylla var. yunnanensis at Different Nitrogen Levels  

作　　者：Can Huang Shubiao Qian Xiaoxian Li Xiahong He Shuhui Zi Congfang Xi Rui Shi Tao Liu 

机构地区：[1]College of Agriculture and Biotechnology,Yunnan Agricultural University,Kunming,Yunnan,650201,China [2]State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan,National&Local Joint Engineering Research Center on Gemplasm Innovation&Utilization of Chinese Medicinal Materials in Southwest China,Yunnan Agricultural University,Kunming,Yunnan,650500,China [3]Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan,650201,China [4]Southwest Forestry University,Kunming,Yunnan,650224,China [5]Guangxi Subtropical Crops Research Institute,Nanning,Guangxi,530001,China

出　　处：《Journal of Botanical Research》2023年第3期1-26,共26页植物研究（英文）

基　　金：supported by the Key R&D Program of Yunnan Province,China(Grant No.202103AC100003;202101AS070228);the Major Spe­cial Project of the Ministry of Science and Technology(2021YFD1000202;2021YFD1601003);and the Na­tional Natural Science Foundation of China(Grant No.31860075),thank you.

摘　　要：Arbuscular mycorrhizal fungi(AMF)are important members of the plant microbiome and affect the uptake and transfer of mineral elements by forming a symbiotic relationship with plant roots.Nitrogen(N),as an important min­eral element,can directly affect plant growth and development at different N levels.It has been confirmed that inoc­ulation with AMF can improve the efficiency of N utilization by plants.However,there are still fewer reports on the dynamic relationship between arbuscular mycorrhizal and plant secondary metabolites at different nitrogen levels.In this experiment,the physiological indexes and genes related to saponin synthesis were determined by applying differ­ent concentration gradients of nitrogen to the medicinal plant P.polyphylla var.yunnanensis infested by AMF as the test material.It was found that nitrogen addition increased the biomass,chlorophyll content,and nutrient content of above-and below-ground plant parts and increased the content of saponin content of P.polyphylla var.yunnanensis to some extent,but AMF inoculation increased the saponin content of P.polyphylla var.yunnanensis more significantly.AMF inoculation also promoted the expression of genes related to the saponin synthesis pathway,including 3-hy­droxy-3-methylglutaryl coenzyme A synthase(HMGS),squalene epoxidase 1(SE1),and cycloartenol synthase(CAS),which is in according with the accumulation of saponin in plants.It also may increase the saponin content of AMF plants by altering the expression of P450s and UGTs related to saponin synthesis.

关 键 词：NITROGEN Arbuscular mycorrhizal fungi SAPONIN P.polyphylla var.yunnanensis 

分 类 号：O62[理学—有机化学]