长非编码RNA LOC285194在宫颈癌中的表达及相关ceRNA网络的探究  

Exploration on the Expression Level of Long Non-coding RNA LOC285194 in Cervical Cancer and the Relative Competing Endogenous RNA Network

作　　者：黄秋霞 欧海玲[1] 黄克强[1] 刘姗姗[1] HUANG Qiuxia;OU Hailing;HUANG Keqiang(Department of Pathology,the First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning City,Guangxi 530023)

机构地区：[1]广西中医药大学第一附属医院病理科,广西南宁市530023

出　　处：《医学理论与实践》2023年第14期2356-2360,2367,共6页The Journal of Medical Theory and Practice

基　　金：广西壮族自治区卫生和计划生育委员会科研课题(Z2016658)。

摘　　要：目的:利用多中心样本探究长非编码RNA LOC285194在宫颈癌组织中的表达水平及相关内源竞争RNA网络。方法:收集来自GEO、TCGA、ArrayExpress、SRA等数据库中与宫颈癌相关的高通量数据集,利用散点图和独立样本t检验探究各个数据集中LOC285194在宫颈癌组织与非癌宫颈组织对照中的表达差异。同时,合并计算标准化均数差(SMD)以综合评估LOC285194在宫颈癌组织中的表达水平。DIANA-LncBase v3和TargetScan Human v8.0数据库用于预测与LOC285194有靶向关系的miRNA以及相关的mRNA,ENCORI数据库用于计算miRNA与相应靶基因表达的相关性。结果:通过综合计算618例样本(CC样本399例,正常宫颈样本219例)的SMD,结果提示LOC285194在宫颈癌组织中的表达水平显著低于正常对照组织(SMD=-0.43,95%CI-0.61~-0.25,P<0.05)。结合预测以及综合计算SMD,3个与LOC285194相关的miRNA被纳入:hsa-miR-130b-5p、hsa-miR-141-5p和hsa-miR-200a-5p。同时,通过综合计算,hsa-miR-141-5p在宫颈癌组织中的高表达及其靶基因ZNF385D的低表达得到了验证。结论:LOC285194在宫颈癌组织中呈显著的低表达,其可能通过相关的内源竞争RNA机制参与宫颈癌的发生与发展,值得进一步的研究探讨。Objective:To investigate the expression level of long non-coding RNA(lncRNA)LOC285194 in cervical cancer(CC)and the relative competing endogenous RNA(ceRNA)network.Methods:The high-throughput datasets related to CC were collected from GEO,TCGA,ArrayExpress and SRA databases.Scatter plots and Student’s t-test were utilized to explore the difference of LOC285194 expression in CC tissues and corresponding normal controls.Meanwhile,an integrated calculation of standard mean difference(SMD)was conducted to assess the expression level of LOC285194 in CC tissues comprehensively.Online tools IANA-LncBase v3 and TargetScan Human v8.0 was utilized to identify LOC285194-related miRNAs and relative target mRNAs.And ENCORI database was used to study the correlations between miRNAs and relative target genes.Results:The pooled SMD of-0.43(95%CI-0.61~-0.25,P<0.05)indicated that the expression of LOC285194 in CC tissues was lower than that in normal controls via integrated study with 618 cases(399 cases of CC,219 cases of normal cervix).Three LOC285194-related miRNAs were included with combination of prediction and integrated SMD(hsa-miR-130b-5p,hsa-miR-141-5p and hsa-miR-200a-5p).Simultaneously,the upregulation of hsa-miR-141-5p and the downregulation of its target ZNF385D in CC were validated by calculation of combined SMD with multicenter samples.Conclusion:This study demonstrated that LOC285194 was significantly downregulated in CC tissues with multicenter samples.It may participate the tumorigenesis and development of CC via relative ceRNA mechanism,which is worthy of further exploration.

关 键 词：宫颈癌 长非编码RNA LOC285194 内源竞争RNA 

分 类 号：R737.33[医药卫生—肿瘤] R36[医药卫生—临床医学]