Trojan nanobacteria hybridized with prodrug nanocapsules for efficient combined tumor therapy  

作　　者：Mingsong Zang Yuancheng Ji Xiaoran Ding Zhengwei Xu Jinxing Hou Jianxin Sun Jiayun Xu Shuangjiang Yu Hongcheng Sun Tingting Wang Junqiu Liu 

机构地区：[1]State Key Laboratory of Supramolecular Structure and Materials,College of Chemistry,Jilin University,Changchun 130012,China [2]Key Laboratory of Organosilicon Chemistry and Material Technology of Ministry of Education,Key Laboratory of Organosilicon Material Technology of Zhejiang Province,College of Material,Chemistry and Chemical Engineering,Hangzhou Normal University,Hangzhou 311121,China [3]Department of Biomedical Engineering,College of Design and Engineering,National University of Singapore,Singapore 117583,Singapore

出　　处：《Nano Research》2023年第7期9651-9662,共12页纳米研究（英文版）

基　　金：This work was supported by the National Key Research and Development Program of China(Nos.2020YFA0908500 and 2018YFA0901600);the National Natural Science Foundation of China(Nos.22275046,22161142015,22075065,22001054,and 22201058);the Hangzhou Leading Innovation and Entrepreneurship Team Project(No.TD2022001);the startup fund from Hangzhou Normal University(No.2019QDL026).

摘　　要：Live bacteria-based drug delivery systems have been raised as promising tools for enhancing drug delivery into tumors due to their active tumor targeting and easy surface modifiability.In this work,a“Trojan nanobacteria hybrid”,E.coli@highly integrated nanocapsules(HINCs)hybrid(HINE-Hybrid),was successfully constructed with HINCs of prodrug based on covalent selfassembly and the facultative anaerobic bacterium E.coli MG 1655 for combined chemotherapy,photothermal therapy(PTT),and chemodynamic therapy(CDT).HINCs were constructed by covalent cross-linking of pillar[5]arene derivatives and cisplatin prodrug linker,which can be endocytosed and lysed to release therapeutic agents.Under the near-infrared(NIR)light(at 808 nm)irradiation,the system temperature can be significantly increased by HINCs,which further leads to the highly efficient generation of reactive oxygen species(ROS).In addition,HINE-Hybrid shows significant antitumor effects in in vitro and in vivo studies and also promotes immune cell infiltration and antitumor cytokine expression in the tumor microenvironment(TME).HINEHybrid exerts its anticancer properties efficiently due to selective enrichment and multiplication of E.coli at tumor sites,which is important for the construction of bacterial-assisted antitumor platforms.

关 键 词：live bacteria hybrid covalent nanocapsule photothermal therapy chemodynamic therapy 

分 类 号：R730.5[医药卫生—肿瘤] TB383[医药卫生—临床医学]