霍山石斛茎段培养诱导丛生芽的研究  

Study on the Induction of Cluster Buds by Stem Segment Culture of Dendrobium huoshanense

作　　者：程贵兰[1] 詹立平 蔡智军[1] 贾金辉[1] 张良英 CHENG Guilan;ZHAN Liping;CAI Zhijun;JIA Jinhui;ZHANG Liangying(Liaoning Agricultural Technical College,Yingkou 115009,China;Liaoning Vocational College of Medicine,Shenyang 1110101,China)

机构地区：[1]辽宁农业职业技术学院,辽宁营口115009 [2]辽宁医药职业学院,辽宁沈阳110101

出　　处：《辽宁农业职业技术学院学报》2023年第4期1-4,14,共5页Journal of Liaoning Agricultural Technical College

基　　金：辽宁农业职业技术学院2019年科研计划项目(Lnz201909)。

摘　　要：以霍山石斛2a生嫩茎为外植体,开展霍山石斛茎段离体培养诱导丛生芽的研究。结果表明:在2.5%的次氯酸钠溶液消毒10min的处理效果较好,污染率26.67%,死亡率23.33%;茎段腋芽在MS、N_(6)培养基上萌发率均能达到90%,优于1/2MS、1/2N_(6);5%土豆泥与10%香蕉泥的组合,添加在MS、N_(6)培养基中,促进腋芽生长要优于仅添加一种有机物;在MS+5%土豆泥+10%香蕉泥的培养基上调整,0.7mg/L6-BA与0.07mg/L NAA的激素配比对霍山石斛丛生芽继代增殖作用突出,增殖倍数在4.2,且丛生芽长势良好。综合分析,最终筛选出霍山石斛丛生芽增殖最佳培养基为MS+5%土豆泥+10%香蕉泥+0.7mg/L6-BA+0.07mg/LNAA。Using 2-year-old tender stems of Dendrobium huoshanense as explants,the study on the induction of cluster buds in vitro culture of stem segments of Dendrobium huoshanense was carried out.The results showed that the treatment effect of 2.5% sodium hypochlorite solution for 10 min was better,the pollution rate was 26.67%,and the mortality rate was 23.33%.The germination rate of axillary buds on MS and N6 medium could reach 90%,which was beter than that of 1/2MS and 1/2N.The combination of 5%potato puree and 10%banana puree was added to MS and N medium to promote the growth of axillary buds,which was better than adding only one organic matter.Adjusted on the medium of MS+5% mashed potato+10%mashed banana,the hormone ratio of 0.7 mg/L 6-BA and 0.07 mg/L NAA had a prominent effect on the subculture and proliferation of Dendrobium huoshanense cluster buds.The proliferation multiple was 4.2,and the cluster buds grew well.Based on the comprehensive analysis,the best medium for the proliferation of Dendrobium huoshanense was MS+5% mashed potato+10% mashed banana+0.7 mg/L 6-BA+0.07 mg/L NAA.

关 键 词：霍山石斛 茎段 消毒时间 腋芽萌发 丛生芽 增殖倍数 

分 类 号：S567.239[农业科学—中草药栽培]