Microcarriers promote the through interface movement of mouse trophoblast stem cells by regulating stiffness  

作　　者：Zili Gao Jia Guo Bo Gou Zhen Gu Tan Jia Sinan Ma Liyuan Jiang Wenli Liu Lixun Zhou Qi Gu 

机构地区：[1]State Key Laboratory of Membrane Biology,The State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing,100101,PR China [2]Beijing Institute for Stem Cell and Regenerative Medicine,Beijing,100101,PR China [3]University of Chinese Academy of Sciences,Beijing,100049,PR China [4]Department of Chemistry and Biological Engineering,University of Science and Technology,Beijing,100083,PR China [5]School of Life Sciences,Northeast Agricultural University,Harbin,150030,PR China

出　　处：《Bioactive Materials》2023年第10期196-205,共10页生物活性材料（英文）

基　　金：supported by National Natural Science Foundation of China(T2222029 and U21A20396);Strategic Priority Research Program of Chinese Academy of Sciences(XDA16020802);CAS Project for Young Scientists in Basic Research(YSBR-012);CAS Engineering Laboratory for Intelligent Organ Manufacturing(KFJ-PTXM-039).

摘　　要：Mechanical force is crucial in the whole process of embryonic development.However,the role of trophoblast mechanics during embryo implantation has rarely been studied.In this study,we constructed a model to explore the effect of stiffness changes in mouse trophoblast stem cells(mTSCs)on implantation:microcarrier was prepared by sodium alginate using a droplet microfluidics system,and mTSCs were attached to the microcarrier surface with laminin modifications,called T(micro).Compared with the spheroid,formed by the self-assembly of mTSCs(T(sph)),we could regulate the stiffness of the microcarrier,making the Young’s modulus of mTSCs(367.70±79.81 Pa)similar to that of the blastocyst trophoblast ectoderm(432.49±151.90 Pa).Moreover,T(micro)contributes to improve the adhesion rate,expansion area and invasion depth of mTSCs.Further,T(micro)was highly expressed in tissue migration-related genes due to the activation of the Rho-associated coiled-coil containing protein kinase(ROCK)pathway at relatively similar modulus of trophoblast.Overall,our study explores the embryo implantation process with a new perspective,and provides theoretical support for understanding the effect of mechanics on embryo implantation.

关 键 词：BIOMECHANICS MICROCARRIERS Embryo implantation Cell migration 

分 类 号：R318[医药卫生—生物医学工程]