间充质干细胞的细胞治疗策略研究进展  

Progress on the cell therapy strategy of mesenchymal stem cells

作　　者：段星祥 张瑞 贺燕 彭友俭[3] 叶青松 DUAN Xingxiang;ZHANG Rui;HE Yan;PENG Youjian;YE Qingsong(Department of Regenerative Medicine,Renmin Hospital of Wuhan University,Wuhan 430060,China;Institute of Regenerative and Translational Medicine,Tianyou Hospital of Wuhan University of Science and Technology,Wuhan 430064,China;Department of Stomatology,Renmin Hospital of Wuhan University,Wuhan 430060,China;Department of Oral and Maxillofacial Surgery,Massachusetts General Hospital and Harvard School of Dental Medicine,Boston 02114,USA)

机构地区：[1]武汉大学人民医院再生医学中心,湖北武汉430060 [2]武汉科技大学附属天佑医院再生与转化医学研究所,湖北武汉430064 [3]武汉大学人民医院口腔医学中心,湖北武汉430060 [4]哈佛大学麻省总医院,哈佛大学牙学院口腔颌面外科,美国波士顿02114

出　　处：《口腔疾病防治》2023年第10期745-750,共6页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金面上项目(81871503);湖北省重点研发计划项目(2022BCA029)。

摘　　要：间充质干细胞(mesenchymal stem cells,MSCs)的细胞治疗是近年研究热点,包括传统意义上的以活细胞为基础的细胞治疗策略和新出现的以细胞外囊泡和其他可溶性蛋白质或生物活性分子为基础的去细胞治疗策略。目前,MSCs诱导获得的细胞已具备成熟的功能和特异性的结构,结合生物材料或类器官技术的原位移植在定植率及功能性方面有了较大提升。另一方面,随着大规模培养技术和细胞外囊泡分离技术的成熟,大量获得高纯度的细胞外囊泡难度降低,细胞外囊泡的研究也逐渐成为当前研究的热点。越来越多的研究支持MSCs的治疗效果不仅是通过植入和分化效应进行修复,MSCs旁分泌也表现出良好效果。笔者对MSCs的细胞治疗策略和去细胞治疗策略的最新进展进行综述。Cell therapy based on mesenchymal stem cells(MSCs)has been a hot research topic in recent years,including the traditional cell therapy strategy based on living cells and the new cell-free therapy strategy based on soluble proteins or bioactive molecules such as extracellular vesicles(EVs).At present,MSC-induced cells have mature functions and specific structures,and insitu transplantation combined with biomaterials or organic technology has greatly improved the settlement rate and function.On the other hand,as the large-scale culture technique and EVs separation technique evolve,it is possible to obtain a large number of pure EVs,and EVs are gradually becoming a hot spot of current research.An increasing number of studies have shown that the therapeutic effect of MSCs not only occurs by implantation and differentiation but also manifests as the paracrine effect of MSCs.In this review,we discuss the emerging outcomes of cell therapies and acellular therapies to alleviate these pathological conditions.

关 键 词：生物材料 再生医学 间充质干细胞 牙髓干细胞 细胞治疗策略 去细胞治疗策略 细胞外囊泡 外泌体 

分 类 号：R78[医药卫生—口腔医学]