CCNB1在宫颈癌中的生物学功能及其与患者预后关系生物信息学分析  

Biological function of CCNB1 in cervical cancer and its relationship with prognosis:a bioinformatics analysis

作　　者：纪霞 郭海荣[1] 王宁 高杰 秦淑丽 杜海燕 JI Xia;GUO Hairong;WANG Ning;GAO Jie;QIN Shuli;DU Haiyan(Baogang Hospital of Inner Mongolia,Baotou 014010,China)

机构地区：[1]内蒙古包钢医院,内蒙古包头014010

出　　处：《包头医学院学报》2023年第7期29-33,共5页Journal of Baotou Medical College

基　　金：包头市科技计划项目(2019Z3010-04)。

摘　　要：目的:采用数据挖掘方法探讨细胞周期蛋白B1(CCNB1)在宫颈癌中的表达、功能富集和信号通路及其与患者预后关系。方法:采用生物信息数据分析方法探讨相关肿瘤数据库中CCNB1基因在宫颈癌组织及其癌旁组织中的表达;对CCNB1基因进行生物学功能和信号通路富集,在STRING数据库中(http://string-db.org/cgi/input.pl)构建CCNB1基因表达蛋白相互作用网络。在Kaplan-Meier数据库(http://kmplot.com/analysis/)中分析CCNB1高低表达与宫颈癌患者总生存(OS)和无疾病进展生存(DFS)的关系。结果:在宫颈癌患者,癌组织CCNB1表达水平明显高于癌旁组织(P<0.05);CCNB1生物学过程、细胞组分和分子功能主要富集于细胞分裂和细胞周期、细胞质及细胞周期蛋白依赖性蛋白丝氨酸/苏氨酸激酶活性;KEGG相关信号通路主要包括细胞周期、孕酮介导的卵母细胞成熟和卵母细胞减数分裂;CCNB1及其相互作用蛋白网络,蛋白与蛋白间的相互作用关系为196,平均聚集指数为0.944,蛋白网络相关作用显著(P<0.005);CCNB1高表达宫颈癌患者总生存(HR=1.62,95%CI:1.10-2.62,P<0.05)和无疾病进展生存(HR=3.53,95%CI:1.62-7.69,P<0.05),显著低于低表达者;CCNB1表达水平与B淋巴细胞(r=-0.099,P>0.05),CD8+T细胞(r=-0.036,P>0.05)、CD4+T细胞(r=0.065,P>0.05)、巨噬细胞(r=-0.79,P>0.05)、中性粒细胞(r=0.1,P>0.05)及树突状细胞浸润无关(r=0.8,P>0.05)。结论:CCNB1在宫颈癌中高表达,并参与了宫颈癌的发生发展,其高表达与患者的预后不良有关,可能成为宫颈癌靶向治疗的新靶点。Objective:To explore the expression,functional enrichment and signal pathway of CCNB1 in cervical cancer and its relationship with patients’prognosis.Methods:The expression of CCNB1 in cervical cancer and its adjacent tissues was analyzed with bioinformatics data analysis.The biological function and signal pathway of CCNB1 were enriched,and the protein interaction network of CCNB1 expression was constructed in the STRING database.The relationship between CCNB1 expression and overall survival(OS)and disease free survival(DFS)was analyzed using Kaplan Meier database.Results:The expression level of CCNB1 in cervical cancer tissues was significantly higher than that in adjacent tissues(P<0.05).The biological processes,cell components and molecular functions of CCNB1 are mainly enriched in cell division and cell cycle,and the activity of serine/threonine kinase in cytoplasm and cyclin dependent protein.KEGG related signaling pathways mainly include cell cycle,progesterone mediated oocyte maturation and oocyte meiosis.The protein-protein interaction relationship of CCNB1 and its interaction protein network was 196,the average aggregation index was 0.944,the interaction of protein-protein interaction network was significant(P<0.005).The OS(HR=1.62,95%CI:1.10-2.62,P<0.05)and DFS(HR=3.53,95%CI:1.62-7.69,P<0.05)of patients with high expression of CCNB1 were significantly lower than those with low expression of CCNB1.CCNB1 expression was not correlated with B lymphocytes(r=-0.099,P>0.05),CD8+T cells(r=-0.036,P>0.05),CD4+T cells(r=0.065,P>0.05),macrophages(r=-0.79,P>0.05),neutrophils(r=0.1,P>0.05)and dendritic cell infiltration(r=0.8,P>0.05).Conclusion:CCNB1 is highly expressed in cervical cancer,and is involved in the occurrence and development of cervical cancer.Its high expression is related to poor prognosis of patients,which may be a new target for targeted therapy of cervical cancer.

关 键 词：宫颈癌 CCNB1 数据挖掘 功能富集 信号通路 

分 类 号：R737.33[医药卫生—肿瘤]