白藜芦醇对大鼠运动性肝损伤的保护作用  

Protective effect of resveratrol on exercise-induced liver injury in rats

作　　者：胡戈 马欣雨 秦菲 曹建民[4] 董丽[4] 周海涛 华冰[5] HU Ge;MA Xinyu;QIN Fei;CAO Jianmin;DONG Li;ZHOU Haitao;HUA Bing(Changzhou University,Changzhou 213164,China;Beijing Union University,Beijing 100101;Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University,Beijing 100191;Beijing Sport University,Beijing 100084;Beijing Technology and Business University,Beijing 100037)

机构地区：[1]常州大学,江苏常州213164 [2]北京联合大学,北京100101 [3]北京联合大学生物活性物质与功能食品北京市重点实验室,北京100191 [4]北京体育大学,北京100084 [5]北京工商大学,北京100037

出　　处：《中国实验动物学报》2023年第6期763-769,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：北京市教育委员会科技计划项目(KM202211417004);北京联合大学科研项目(ZK70202005);北京联合大学“启明星”大学生科技创新项目(20222010)。

摘　　要：目的探讨白藜芦醇保护大鼠运动性肝损伤的可能机制。方法7周龄雄性SD大鼠32只,SPF级,随机分为安静对照组(C组)、白藜芦醇对照组(R组)、运动性肝损伤模型组(M组)和白藜芦醇干预组(RM组),每组8只。C组、R组不进行运动干预,其余两组进行4周大强度跑台训练(坡度10°,以10 m/min速度起始,每5 min速度递增5 m/min,至35 m/min不再增加,运动至力竭)。每次训练前1 h,R组、RM组灌胃白藜芦醇溶液(150 mg/(kg·bw),5 mL/(kg·bw)),其余两组灌胃等体积溶剂。末次训练结束后24 h,取大鼠血及肝。全血生化仪检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性;HE染色后观察肝组织形态;免疫组化法检测肝核因子E2相关因子2(Nrf2)、血红素氧合酶1(HO-1)、凋亡相关蛋白B淋巴细胞瘤因子-2(Bcl-2)和Bcl-2相关X蛋白(Bax)蛋白质表达水平;TUNEL法检测肝细胞凋亡水平;比色法检测肝超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平。结果与M组相比,RM组大鼠肝损伤标志物血清ALT、AST活性及肝MDA和细胞凋亡水平显著降低(P<0.05或P<0.01),肝Nrf2与HO-1蛋白质表达水平、SOD活性及Bcl-2/Bax比值显著升高(P<0.05或P<0.01),且肝病理性改变有效改善。结论4周白藜芦醇干预可有效改善大鼠运动性肝损伤,其机制可能是通过激活肝Nrf2信号通路,拮抗氧化应激诱导的细胞凋亡过度发生。Objective To investigate the mechanism of resveratrol protecting exercise-induced liver injury in rats.Methods Thirty-two 7-week-old male SPF SD rats were randomly divided into a control group(Group C),resveratrol control group(Group R),exercise-induced liver injury model group(Group M),and resveratrol treatment group(Group RM)with eight rats in each group.Group C and Group R did not undergo any exercise.Group M and Group RM underwent 4 weeks of high-intensity treadmill training(10°slope;velocity started from 10 m/min,which was increased by 5 m/min every 5 min and would not increase until 35 m/min,and then the rats were trained to exhaustion).One hour before training,Group R and Group RM were intragastrically injected with 150 mg/(kg·bw)resveratrol in 5 mL/(kg·bw).Group C and Group M were injected with an equal volume of solvent at the same time point.Rats were sacrificed at 24 hours after the end of the last training session,and blood and liver were collected.Serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activity were detected by an automatic biochemical analyzer.Hepatic histomorphology was observed by hematoxylin-eosin(HE)staining.Hepatic nuclear factor-E2-related factor 2(Nrf2),heme oxygenase-1(HO-1),apoptosis-related protein B-cell lymphoma-2(Bcl-2),and bcl-2-associated X protein(Bax)protein expression was detected by immunohistochemistry.Hepatic apoptosis was detected by TdT-mediated dUTP nick-end labeling.Hepatic superoxide dismutase(SOD)and malondialdehyde(MDA)activity was detected by colorimetry.Results Compared with Group M,serum ALT,AST activity,the hepatic MDA level,and hepatic apoptosis were significantly decreased in Group RM(P<0.05 or P<0.01),hepatic Nrf2 and HO-1 protein expression,SOD activity,the and Bcl-2/Bax ratio were increased significantly(P<0.05 or P<0.01),and liver histopathological changes had effectively improved.Conclusions Four weeks of resveratrol treatment alleviates exercise-induced liver injury in rats,and the mechanism of its protective effect might be r

关 键 词：白藜芦醇 大强度运动 肝损伤 大鼠 核因子E2相关因子2信号通路 

分 类 号：Q95-33[生物学—动物学]