缺血性脑卒中凋亡相关lncRNA-mRNA网络构建及核心基因预测  

Construction of apoptosis-related lncRNA-mRNA network in ischemic stroke and prediction of hub genes

作　　者：古联[1] 杨怡冰 严雁[1] 黄先利 苏莉[3] 梁宝云 GU Lian;YANG Yibing;YAN Yan;HUANG Xianli;SU Li;LIANG Baoyun(Ward Two of Encephalopathy,the First Affiliated Hospital of Guangxi University of Chinese Medicine,Guangxi Zhuang Autonomous Region,Nanning530022,China;Graduate School,Guangxi University of Chinese Medicine,Guangxi Zhuang Autonomous Region,Nanning530001,China;Public Health School,Guangxi Medical University,Guangxi Zhuang Autonomous Region,Nanning530021,China)

机构地区：[1]广西中医药大学第一附属医院脑病二区,广西南宁530022 [2]广西中医药大学研究生院,广西南宁530001 [3]广西医科大学公共卫生学院,广西南宁530021

出　　处：《中国医药导报》2023年第20期4-9,共6页China Medical Herald

基　　金：国家自然科学基金资助项目(81860822、81473670);广西壮族自治区自然科学基金项目(2018GXNSFDA050005)。

摘　　要：目的 探索与缺血性脑卒中(IS)凋亡相关的长链非编码RNA(lncRNA)-mRNA网络并预测核心基因。方法 选取2016年1月至2017年6月广西中医药大学第一附属医院(以下简称“我院”)收治的10例IS患者和同期我院社区卫生体检中心10名健康对照者作为研究对象。对微阵列表达谱进行差异分析,使用加权基因共表达网络分析筛选目标模块内的基因,并与凋亡基因取交集。运用STRING网站和Cytoscape软件筛选前10位差异mRNAs(DEMs),同时与差异lnc RNAs(DELs)构建lncRNA-mRNA共表达网络,最后筛选核心基因。结果 获得3 930个DEMs,模块基因与凋亡基因取交集后得到了553个与凋亡相关的IS基因。构建的lncRNA-m RNA共表达网络包含9个DEMs、277个DELs,最终筛选出7个核心基因G015366、G068382、PIK3CB、BPTF、NR3C1、Akt2和INS。结论 G015366、G068382、PIK3CB、BPTF、NR3C1、Akt2和INS与IS的发生发展相关,可能成为IS诊断标志物。Objective To explore the apoptosis-related long non-coding RNA(lncRNA)-mRNA network in ischemic stroke(IS)and predict the hub genes.Methods A total of 10 patients with IS admitted to the First Affiliated Hospital of Guangxi University of Chinese Medicine(“our hospital”for short)from January 2016 to June 2017,and 10 healthy controls from community health examination center of our hospital during the same period were selected as the research objects.Differential analysis of microarray expression profiles was performed,weighted gene co-expression network analysis was used to screen out genes in the target module,and the intersection with apoptotic genes was taken.Top 10 differentially mRNAs(DEMs)were selected using the STRING website and Cytoscape software,and lncRNA-mRNA co-expression network was constructed between DEMs and differentially lncRNAs(DELs).Finally,hub genes were obtained.Results A total of 3930 DEMs were obtained,and 553 apoptosis-related IS genes were obtained after intersection of module genes and apoptosis genes.The lncRNA-mRNA co-expression network consisted of 9 DEMs and 277 DELs.Finally,7 hub genes G015366,G068382,PIK3CB,BPTF,NR3C1,Akt2,and INS were obtained.Conclusion G015366,G068382,PIK3CB,BPTF,NR3C1,Akt2,and INS are related to the occurrence and development of IS,and may become diagnostic markers of IS.

关 键 词：缺血性脑卒中 凋亡 MRNA 长链非编码RNA 

分 类 号：R743[医药卫生—神经病学与精神病学]