巨噬细胞移动抑制因子在大鼠蛛网膜下腔出血后早期脑损伤中的作用  

Role of macrophage migration inhibitor in early brain injury after subarachnoid hemorrhage in rats

作　　者：申屠华松 陈亦华[1] 程振宇[1] SHENTU Huasong;CHEN Yihua;CHENG Zhenyu(Department of Neurosurgery,Jinhua People’s Hospital,Zhejiang Province,Jinhua 321000,China)

机构地区：[1]浙江省金华市人民医院神经外科,浙江金华321000

出　　处：《中国医药导报》2023年第21期14-18,共5页China Medical Herald

基　　金：浙江省金华市科技计划项目(2021-4-063);浙江省医药卫生科技计划项目(2020KY1017)。

摘　　要：目的揭示巨噬细胞移动抑制因子(MIF)在大鼠蛛网膜下腔出血(SAH)后早期脑损伤(EBI)中的作用。方法将120只12月龄的健康雄性Sprague Dawley大鼠(清洁级)按照随机数字表法分为假手术(Sham)组、SAH组和抑制剂组,每组40只。手术操作前0.5 h,抑制剂组大鼠腹腔注射MIF抑制剂(10 mg/kg),SAH组大鼠和Sham组大鼠腹腔注射同等剂量0.9%氯化钠注射液,随后采用枕大池二次注血法制作SAH组和抑制剂组大鼠SAH模型,Sham组大鼠模型两次注入的等量液体则为0.9%氯化钠注射液。术后行脑皮层MIF蛋白、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、IL-6、神经细胞凋亡、脑含水量、血脑屏障通透性和神经行为学功能测定。结果与Sham组比较,SAH组大鼠MIF蛋白、IL-1β、TNF-α、IL-6、凋亡神经细胞比例、脑含水量指数和伊文氏蓝含量显著升高,神经行为学评分显著下降(P<0.05);与SAH组比较,抑制剂组大鼠IL-1β、TNF-α、IL-6、凋亡神经细胞比例、脑含水量指数和伊文氏蓝含量显著下降,神经行为学评分显著升高(P<0.05),而SAH组和抑制剂组大鼠MIF蛋白水平比较,差异无统计学意义(P>0.05)。结论MIF可能诱发大鼠SAH后EBI,而抑制MIF作用可能对EBI起到保护作用。Objective To investigate the role of macrophage migration inhibitory factor(MIF)in early brain injury(EBI)after subarachnoid hemorrhage(SAH)in rats.Methods A total of 12012-month-old healthy male Sprague Dawley rats(clean grade)were divided into Sham group,SAH group and inhibitor group according to random number table method,with 40 rats in each group.0.5 h before operation,rats in the inhibitor group were intraperitoneally injected with MIF inhibitor(10 mg/kg),and rats in the SAH group and Sham group were intraperitoneally injected with 0.9%sodium chloride injection,and then SAH group and inhibitor group were constructed by secondary blood injection in the occipital cisterna.In the Sham group,0.9%sodium chloride injection was injected with the same amount of liquid twice.Cortical MIF protein,interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),IL-6,nerve cell apoptosis,brain water content,blood-brain barrier permeability and neurobehavioral function were determined after surgery.Results Compared with Sham group,MIF protein,IL-1β,TNF-α,IL-6,proportion of apoptotic nerve cells,brain water content index and content of Evan’s blue of rats in SAH group were significantly increased,and neurobehavioral scores were significantly decreased(P<0.05).Compared with SAH group,IL-1β,TNF-α,IL-6,proportion of apoptotic nerve cells,brain water content index and content of Evan’s blue in inhibitor group were significantly decreased,and neurobehavioral scores were significantly increased(P<0.05),but there was no statistical significance in MIF protein level between SAH group and inhibitor group(P>0.05).Conclusion MIF may induce EBI after SAH,and inhibition of MIF may protect EBI.

关 键 词：巨噬细胞移动抑制因子 大鼠 蛛网膜下腔出血 早期脑损伤 机制 

分 类 号：R743.35[医药卫生—神经病学与精神病学]