Securin对神经胶质瘤细胞系U251迁移、侵袭以及上皮-间充质转化的影响  

Effects of securin on the migration,invasion,and epithelial mesenchymal transformation of glioma cell line U251

作　　者：王薇[1] 窦佳 李超 刘淑娟 闫海成[2] WANG Wei;DOU Jia;LI Chao;LIU Shujuan;YAN Haicheng(Department of Medical Oncology,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050;Department of Neurosurgery,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院肿瘤内科,呼和浩特010010 [2]内蒙古医科大学附属医院神经外科,呼和浩特010010

出　　处：《临床与病理杂志》2023年第5期849-856,共8页Journal of Clinical and Pathological Research

基　　金：国家自然科学基金(81660469);内蒙古自然科学基金(2020LH08035);内蒙古卫生健康科技计划项目(202202191);内蒙古医科大学附属医院青年骨干项目(2022NYFYFG023)。

摘　　要：目的:探讨securin对神经胶质瘤迁移、侵袭以及上皮-间充质转化的影响。方法:利用在线基因表达谱交互分析(Gene Expression Profiling Interactive Analysis,GEPIA)2.0和Human Protein Atlas数据库分析securin基因和蛋白在神经胶质瘤中的表达情况。在胶质瘤U251细胞中转染干扰securin表达的小干扰RNA(small interfering RNA,siRNA;si-securin)序列,蛋白质印迹法和real-time RT-PCR分别检测U251细胞中蛋白和securin信使RNA(messenger RNA,mRNA)表达水平。Transwell实验检测转染si-securin对U251细胞迁移和侵袭影响。Real-time RT-PCR检测转染si-securin对U251细胞中snail、E-cadherin、N-cadherin、vimentin、基质金属蛋白酶(matrix metalloproteinase,MMP)-2、MMP-9 mRNA表达的影响。结果:在线数据库GEPIA分析发现:与正常人对照组(normal组)相比,神经胶质瘤患者中securin蛋白和mRNA的表达水平均显著上调(均P<0.001)。蛋白质印迹法和real-time RT-PCR检测结果显示:与转染siRNA阴性对照(si-negative control,si-NC)组相比,转染si-securin组U251细胞中securin蛋白和mRNA表达水平均显著下调(均P<0.001)。Transwell实验结果发现:与转染si-NC组相比,转染si-securin组U251细胞迁移和侵袭数均显著减少(均P<0.001)。Real-time RT-PCR检测结果发现:与转染si-NC组相比,转染si-securin组U251细胞中snail、N-cadherin、vimentin、MMP-2、MMP-9蛋白和mRNA相对表达水平均显著降低(均P<0.001),而E-cadherin蛋白和mRNA相对表达水平却显著上调(P<0.001)。结论:干扰securin表达可抑制神经胶质瘤细胞系U251的迁移、侵袭以及上皮-间充质转化。Objective:To investigate the effects of securin on glioma migration,invasion,and epithelial-mesenchymal transformation.Methods:The expression of securin gene in glioma was analyzed using the online Gene Expression Profiling Interactive Analysis(GEPIA)2.0 and Human Protein Atlas database.The small interfering RNA(siRNA)sequence that interfered the securin expression(sisecurin group)was transfected into glioma U251 cells.The mRNA and protein expression levels of securin in U251 cells were detected by real-time RT-PCR and Western blotting,respectively.The effects of transfection of si-securin on migration and invasion of U251 cells were detected by Transwell assay.The effects of transfection of si-securin on the mRNA expression of snail,E-cadherin,N-cadherin,vimentin,matrix metalloproteinase(MMP)-2,MMP-9 in U251 cells were detected by real-time RT-PCR.Results:GEPIA online database analysis showed that the expression level of securin in glioma patients was significantly higher than that in the normal group(both P<0.001).Real-time RT-PCR and Western blotting results showed that the mRNA and protein expression levels of securin in the si-securin group were significantly lower than those in the si-negative control(si-NC)group(both P<0.001).Transwell assay results show that the number of migration and invasion of U251 cells in the si-securin group were both significantly lower than that in the si-NC group(both P<0.001).Real-time RT-PCR results showed that the relative mRNA and protein expression levels of snail,E-cadherin,N-cadherin,vimentin,MMP-2,MMP-9 in the si-securin group were significantly lower than those in the si-NC group(all P<0.001),while the mRNA and protein relative expression level of E-cadherin was significantly higher(P<0.001).Conclusion:Interfering with securin expression suppresses the migration,invasion,and epithelial mesenchymal transformation of glioma cell line U251.

关 键 词：神经胶质瘤 SECURIN 迁移 侵袭 上皮-间充质转化 

分 类 号：R739.4[医药卫生—肿瘤]