Volatile metabolome and transcriptome reveal fragrance release rhythm and molecular mechanisms of Rosa yangii  

作　　者：ZHOU Li-jun HUANG Run-huan LIU Ting-han LIU Wei-chao CHEN Yun-yi LÜPei-feng LUO Le PAN Hui-tang YU Chao ZHANG Qi-xiang 

机构地区：[1]Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding/National Engineering Research Center for Floriculture/Beijing Laboratory of Urban and Rural Ecological Environment/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education/School of Landscape Architecture,Beijing Forestry University,Beijing 100083,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第7期2111-2125,共15页农业科学学报（英文版）

基　　金：supported by the Beijing High-Precision Discipline Project,Discipline of Ecological Environment of Urban and Rural Human Settlements and the National Key R&D Program of China(2019YFD1000400)。

摘　　要：Rose is a highly significant ornamental plant with substantial edible and medicinal value,cultivated worldwide primarily for perfume production.Recently,Rosa yangii,a new species found in northwestern Yunnan,China,has drawn attention due to its strong sweet scented flowers.In this study,the floral components of R.yangii were extracted at different flowering stages using solid phase micro extraction(SPME)and analyzed through gas chromatography-mass spectrometry(GC-MS).A total of 131 volatile organic compounds(VOCs)were detected from R.yangii,including 69 odor compounds.The production and release of floral VOCs were the highest during the initial-open stage,making it the most suitable time for harvesting as a significant number of floral components were synthesized and preserved.The analysis of the odor activity values(OAV)highlighted several key aromatic ingredients of R.yangii,such as eugenol,methyleugenol,benzeneacetaldehyde and phenylethylalcohol,heptanal,decanal,(E)-2-hexen-1-yl acetate,caryophyllene,and others.Metabolome and time-order gene co-expression networks(TO-GCN)revealed that VOCs and benzenoids/phenylpropanoids,along with associated genes,played a pivotal role in the overall floral regulatory network of R.yangii.MYB and bHLH were identified as the essential regulatory factors governing the regulation of eugenol synthase(EGS)and isoeugenol synthase(IGS),consequently influencing the sweet scent of R.yangii.The findings of this study provide a scientific foundation for enhancing fragrance through molecular breeding of ornamental plants.Furthermore,the study facilitated the development and utilization of this new plant’s essential oil material in various industries,including food storage,aromatherapy,cosmetic,and perfumery.

关 键 词：rose sweet fragrance SPME-GC-MS aroma VOCs TO-GCN 

分 类 号：S685.12[农业科学—观赏园艺]