植物乳杆菌对荔枝叶青贮品质及体外降解特性的影响  

The Effects of Lactobacillus Plantarum on Fermentation Quality and In Vitro Digestion Characteristics of Litchi Leaves Silage

作　　者：陈丹丹 黄佩珊 张超[1] 陈晓阳[1] 周玮[1] 张庆[1,2] CHEN Dan-dan;HUANG Pei-shan;ZHANG Chao;CHEN Xiao-yang;ZHOU Wei;ZHANG Qing(South China Agricultural University,Guangzhou,Guangdong Province 510642,China;Maoming Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Maoming,Guangdong Province 525000,China)

机构地区：[1]华南农业大学,广东广州510642 [2]岭南现代农业科学与技术广东省实验室茂名分中心,广东茂名525000

出　　处：《草地学报》2023年第7期2232-2240,共9页Acta Agrestia Sinica

基　　金：广东省自然基金项目(2020A1515011253);广州市科技计划项目(202102020808);茂名实验室茂名实验室自主科研项目(2022ZD002)资助。

摘　　要：为探究发展荔枝叶作为青贮饲料的可行性,4种荔枝叶(‘晚埔’‘乌叶舅’‘桶仔’‘状元红’)分别用植物乳杆菌(Lactobacillus plantarum,LP)处理并设对照组。青贮3,7,14,30天后分析其发酵品质并测定30天后荔枝叶的干物质消化率和累积产气量。结果表明:添加LP降低了4种荔枝叶的pH值和大肠杆菌数量,同时减少了氨态氮产生(P<0.01)。此外LP处理的‘晚埔’和‘状元红’荔枝叶中酵母菌数量比对照低。青贮30天后,LP处理的‘桶仔’和‘状元红’荔枝叶中霉菌数量比对照组低,且低于检测水平。添加LP增加了4种荔枝叶的体外干物质消化率;降低(P<0.01)了产气量。本研究表明,LP添加有助于改善4荔枝叶的发酵质量以及体外发酵特性,发展荔枝叶作为青贮原料可能是回收荔枝叶的一种可行方式。Due to the restricting factor of feed shortage,making full use of natural woody plant resources as animal feed is becoming an extremely important strategy in the tropic.Using litchi leaves as an unconventional feed might meet the requirement of sustainable and intensive livestock production to reduce feed costs,avoid food competition between humans and animals,and promote feed supplies.Therefore,to investigate the feasibility of developing litchi leaves as silage,we investigated the fermentation quality of four varieties of litchi leaves(‘Wanpu’‘Wuyejiu’‘Tongzai’and‘Zhuangyuanhong’)ensiled with or without Lactobacillus plantarum(LP)on day 3,7,14 and 30.The in vitro dry matter digestibility(IVDMD)and accumulated gas production of litchi leaves silages were also investigated after 30 days of ensiling.The results showed that LP inclusive into the ensilage significantly reduced pH value,inhibited the coliform bacteria,and reduced the production of ammonia nitrogen of litchi leaves silage of the four varieties(P<0.01).Moreover,LP treated litchi leaves(‘Wanpu’and‘Zhuangyuanhong’)had lower yeasts than the untreated litchi leaves during ensiling.The number of molds in LP treated groups(‘Tongzai’and‘Zhuangyuanhong’)was below the detected level after 30 days ensiling,which was lower than that of the untreated groups.The addition of LP also contributed to the IVDMD improving and markedly reduction(P<0.01)of gas production of all litchi leaves silages.It was concluded in this study that LP can improve the fermentation quality and in vitro digestion characteristics of litchi leaves silage;developing litchi leaves as silage material is a feasible way to recycle litchi leaves.

关 键 词：荔枝叶 植物乳杆菌 发酵 消化率 产气量 

分 类 号：S816.53[农业科学—饲料科学]