Dietary Epimedium extract supplementation improves intestinal functions and alters gut microbiota in broilers  

作　　者：Jiaqi Zhang Haitao Yu Huiyan Zhang Qingyu Zhao Wei Si Yuchang Qin Junmin Zhang 

机构地区：[1]State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,100193 Beijing,China [2]Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs,Institute of Animal Sciences of Chinese Academy of Agricultural Sciences,100193 Beijing,China.

出　　处：《Journal of Animal Science and Biotechnology》2023年第4期1584-1597,共14页畜牧与生物技术杂志（英文版）

基　　金：supported by The National Key Research and Development Program of China(2021YFD1300301/2021YFD1300303);The Special Basic Research Fund for Central Public Research Institutes(2021-YWF-ZYSQ-06);The Agricultural Science and Technology Innovation Program(ASTIP)in CAAS and its key projects(CAAS-ZDRW202111);The Chinese Academy of Agricultural Science and Technology Innovation Project(ASTIP-IAS-12)。

摘　　要：Background Growth-promoting antibiotics have been banned by law in the livestock and poultry breeding industry in many countries.Various alternatives to antibiotics have been investigated for using in livestock.Epimedium(EM)is an herb rich in flavonoids that has many beneficial effects on animals.Therefore,this study was planned to explore the potential of EM as a new alternative antibiotic product in animal feed.Methods A total of 7201-day-old male broilers(Arbor Acres Plus)were randomly divided into six groups and fed basal diet(normal control;NC),basal diet supplemented with antibiotic(75 mg/kg chlortetracycline;CTC),and basal diet supplemented with 100,200,400 or 800 mg/kg EM extract for 6 weeks(EM100,EM200,EM400 and EM800 groups).The growth performance at weeks 3 and 6 was measured.Serum,intestinal tissue and feces were collected to assay for antioxidant indexes,intestinal permeability,lactic acid and short-chain fatty acids(SCFAs)profiles,microbial composition,and expression of intestinal barrier genes.Results The average daily feed intake in CTC group at 1-21 d was significantly higher than that in the NC group,and had no statistical difference with EM groups.Compared with NC group,average daily gain in CTC and EM200 groups increased significantly at 1-21 and 1-42 d.Compared with NC group,EM200 and EM400 groups had significantly decreased levels of lipopolysaccharide and D-lactic acid in serum throughout the study.The concentrations of lactic acid,acetic acid,propionic acid,butyric acid and SCFAs in feces of birds fed 200 mg/kg EM diet were significantly higher than those fed chlortetracycline.The dietary supplementation of chlortetracycline and 200 mg/kg EM significantly increased ileal expression of SOD1,Claudin-1 and ZO-1 genes.Dietary supplemented with 200 mg/kg EM increased the relative abundances of g_NK4A214_group and Lactobacillus in the jejunal,while the relative abundances of Microbacterium,Kitasatospora,Bacteroides in the jejunal and Gallibacterium in the ileum decreased.Conclusion Supplementati

关 键 词：ANTIOXIDATION BROILERS EPIMEDIUM Growth performance MICROBIOTA 

分 类 号：S831.5[农业科学—畜牧学]