Active bleb formation is abated in Lytechinus variegatus red spherule coelomocytes after disruption of acto-myosin contractility  

作　　者：Lisanne D’ANDREA-WINSLOW Amy K.NOVITSKI 

机构地区：[1]Department of Biology,Northwestern College,St.Paul,Minnesota,USA

出　　处：《Integrative Zoology》2008年第2期115-122,共8页整合动物学（英文版）

摘　　要：Red spherule coelomocytes are immune cells in the sea urchin Lytechinus variegatus that have been characterizedas motile O2 transport cells. Video microscopy of living red spherule coelomocytes reveals a constitutive, dynamicarray of cellular morphologies and movements. Cells continuously send out and retract membrane blebs all over thecell surface as part of their normal cellular physiology. Disruption of microtubules by perfusion with either nocodazoleor taxol had no effect on bleb formation or motility. Perfusion with cytochalasin B abated bleb formation andrevealed cells that exhibited multiple small spheres attached by short membrane extensions. Attenuation of blebbingand intracellular organelle motility were restored by washing out with cytochalasin B. Treatment with phalloidinalso abated bleb formation and revealed a smooth, spherical cellular morphology. The effects of phalloidin werecompletely reversible after washout. Red spherule coelomocytes treated with blebbistatin rounded up with anirreversible retraction of blebs into surface blebs that were greatly reduced in size, number and motility. Normal cellsurface bleb formation and intracellular organelle motility were not restored after washout of the drug. These resultsindicate that the acto-myosin contractile mechanism contributes to the dynamics of constitutive cell surface membraneblebbing in invertebrate immune cells.

关 键 词：sea urchin invertebrate immunology COELOMOCYTE cell motility cell movement microfilament dynamics 

分 类 号：R73[医药卫生—肿瘤]