锶和氮改性二氧化钛/纳米羟基磷灰石复合树脂的制备和性能  

Preparation and properties of strontium and nitrogen co-doped titanium dioxide/nano hydroxyapatite composites resins

作　　者：赵远航 闫琳琳 宋嘉卓 邹馨颖 赵红 刘新 陈佳文 于依岩 张志民[1] 张红[1] ZHAO Yuanhang;YAN Linlin;SONG Jiazhuo;ZOU Xinying;ZHAO Hong;LIU Xin;CHEN Jiawen;YU Yiyan;ZHANG Zhimin;ZHANG Hong(Department of Endodontics,Stomatology Hospital,Jilin University,Changchun 130021,China;Jilin Provincial International Cooperation Base of Oral Biomedicine,Changchun 130021,China)

机构地区：[1]吉林大学口腔医院牙体牙髓科,吉林长春130021 [2]吉林省口腔生物医学国际联合研究中心,吉林长春130021

出　　处：《吉林大学学报（医学版）》2023年第4期1067-1075,共9页Journal of Jilin University：Medicine Edition

基　　金：吉林省财政厅科技项目(JCSZ2020304-2,JCSZ2021893-10)。

摘　　要：目的:制备锶和氮改性二氧化钛(Sr-N-TiO_(2))/纳米羟基磷灰石(n-HA)复合树脂,并对其基础性能、抗菌性能、再矿化能力和生物安全性进行评价,阐明新型复合树脂的抗菌机制。方法:Sr-N-TiO_(2)与n-HA组合作为增强填料,制备复合树脂,分为不含增强填料的对照组和3个实验组,根据实验组新型复合树脂中增强填料质量分数不同又分为2.5%增强填料组、5.0%增强填料组和7.5%增强填料组。采用傅里叶红外吸收光谱分别检测光固化60 s前后各组复合树脂的红外光谱,计算复合树脂双键转化率。使用直径4 mm、高10 mm的模具制备各组复合树脂试件,计算光照20 s后各组复合树脂试件固化深度。每组制备3个复合树脂试件,将各组试件与稀释的变异链球菌液共培养,通过平板菌落计数法检测各组试件表面黏附细菌菌落数并计算抗菌率,采用活/死细菌染色法观察各组复合树脂试件表面活/死细菌比例及形态表现。将各组复合树脂试件于人工唾液中浸泡1、3、5和7 d,采用扫描电子显微镜(SEM)观察各组试件表面再矿化情况。小鼠纤维细胞(L-929细胞)接种于各组复合树脂浸提液中,采用CCK-8法分别于1、2和3 d时检测各组细胞相对增殖率(RGR),进行细胞毒性等级评价。结果:随着复合树脂中增强填料比例的增加,新型复合树脂双键转化率和固化深度呈降低趋势,但均达到临床使用标准。与对照组比较,2.5%和5.0%增强填料组新型复合树脂双键转化率差异无统计学意义(P>0.05),7.5%增强填料组新型复合树脂双键转化率明显降低(P<0.05)。与对照组比较,各实验组新型复合树脂固化深度明显降低(P<0.05或P<0.01),表面黏附细菌菌落计数明显减少(P<0.01)。当增强填料比例达到5.0%时,新型复合树脂抗菌率大于90%。与对照组比较,各实验组新型复合树脂试件表面活细菌数随增强填料比例升高逐渐减少。SEM观察,各实验组新型Objective:To prepare the strontium and nitrogen co-doped titanium dioxide(Sr-N-TiO_(2))/nano hydroxyapatite(n-HA)composite resins,to evaluate its basic properties,antibacterial properties,remineralization abilities,and biosafeties,and to clarify the antibacterial mechanism of the new type of composite resins.Methods:Sr-N-TiO_(2) and n-HA were mixed as the reinforcing fillers.The composite resins were prepared and were divided into control group and three experimental groups.According to the mass fractions of reinforcing fillers,the experimental groups were divided into 2.5%reinforcing filler group,5.0%reinforcing filler group,and 7.5%reinforcing filler group.The infrared spectrogram of composite resins in various groups were detected by the Fourier-transform infrared absorption spectrogram and the double bond conversion rates were calculated before and after light curing for 60 s.The curing depths of composite resin specimens in various groups after light curing for 20 s were calculated by cylindrical mould(height=10.0 mm,diameter=4.0 mm).Three composite resin specimens in various groups were prepared and co-cultured with the dilute Streptococcus mutans solution.The plate colony counting method was used to determine the counts of attached bacteria on surface of the specimens and the antibacterial rates were calculated;live/dead bacteria staining method was used to observe the ratio of live bacteria/dead bacteria and morphology of composite resin speciments in various groups.The composite resin specimens were immersed in the artificial saliva for 1,3,5,and 7 d,and the mineralizations of surface of the composite resins were observed under scanning electron microscope(SEM).The mouse fibroblast cells(L-929 cells)were incubated with the resin extract solution,and CCK-8 method was used to detect the relative growth rates(RGR)of the cells on the 1st,2nd,and 3rd days and the cytotoxicity levels were evaluated.Results:With the increasing of reinforcing filler proportion of the composite resins,the double bond conversion r

关 键 词：复合树脂 龋病 继发龋 抗菌性 再矿化 

分 类 号：R781.1[医药卫生—口腔医学]