沉默RelB下调MnSOD减弱非小细胞肺癌的放疗抵抗  

Silencing of RelB Attenuates Radiotherapy Resistance of Non-Small Cell Lung Cancer by Downregulating MnSOD

作　　者：黄名萱 杨丽琴[2] 秦华龙[2] HUANG Ming-xuan;YANG Li-qin;QIN Hua-long(Suzhou Medical College of Soochow University,Suzhou,Jiangsu,215123,China;Department of Thoracic Surgery,First Affiliated Hospital of Soochow University,Suzhou,Jiangsu,215006,China)

机构地区：[1]苏州大学苏州医学院,江苏苏州215123 [2]苏州大学附属第一医院胸外科,江苏苏州215006

出　　处：《中国血液流变学杂志》2023年第1期11-15,共5页Chinese Journal of Hemorheology

基　　金：苏州大学第二十四批大学生课外学术科研基金项目。

摘　　要：目的探讨核转录因子RelB调控非小细胞肺癌放疗抵抗的机制。方法收集苏州大学附属第一医院胸外科手术切除的Ⅰ-Ⅲa期非小细胞肺癌患者的临床资料和组织标本。免疫组化法分析RelB在癌和癌旁组织中的表达,Chi-square分析RelB与临床参数的相关性。建立稳定沉默RelB的细胞株A549-siRelB,辐照细胞株,流式细胞术检测辐照后细胞株的细胞凋亡,Westernblot检测辐照后细胞株中MnSOD表达。结果RelB在癌组织中的表达高于癌旁组织,RelB的高表达与肿瘤侵犯程度、淋巴结转移及TNM分期有关。辐照后A549-siRelB的细胞凋亡数多于A549-sictrl,A549-siRelB中MnSOD水平较A549-sictrl低。结论核转录因子RelB参与非小细胞肺癌的进展,沉默RelB下调MnSOD减弱非小细胞肺癌的放疗抵抗。Objective To explore the mechanism of nuclear transcription factor RelB regulating the radiotherapy resistance of non-small cell lung cancer.Methods The clinical data and tissue samples of patients with stageⅠ-Ⅲa non-small cell lung cancer resected by thoracic surgery in the First Affiliated Hospital of Soochow University were collected.The expression of RelB in the tumor tissue and paracancerous tissue was examined by immunohistochemistry.The Chi-square test was used to analyze the correlation between RelB expression and clinical parameters.The cell line with stable silencing RelB A549-siRelB was established,followed by irradiation.The cellular apoptosis of irradiated cell lines was detected by flow cytometry.MnSOD expression of irradiated cell lines was determined by Western blotting.Results The expression of RelB was increased in tumor tissue compared with paracancerous tissue.High RelB expression was significantly correlated with the depth of tumor invasion,lymph node metastasis,and tumor-node-metastasis(TNM)classification.After irradiation,the cellular apoptosis rate of A549-siRelB was higher than that of A549-sictrl,and the level of MnSOD of A549-siRelB was lower than that of A549-sictrl.Conclusion The nuclear transcription factor RelB is involved in the progression of non-small cell lung cancer,and silencing RelB attenuated radiotherapy resistance of nonsmall cell lung cancer by downregulating MnSOD.

关 键 词：非小细胞肺癌 RELB 放疗抵抗 

分 类 号：R734.2[医药卫生—肿瘤]