LncRNA-AP006284.1 promotes prostate cancer cell growth and motility by forming RNA-DNA triplexes and recruiting GNL3/SFPQ complex to facilitate RASSF7 transcription  

作　　者：Yali Lu Yan Lin Xiaoyang Zhang Jun Yan Zhe Kong Lu Zhang Chenji Wang Yan Huang Shimin Zhao Yao Li 

机构地区：[1]Obstetrics and Gynecology Hospital,State Key Laboratory of Genetic Engineering,MOE Engineering Research Center of Gene Technology,School of Life Science,Fudan University,Shanghai 200433,China [2]Shanghai Engineering Research Center of Industrial Microorganisms,School of Life Science,Fudan University,Shanghai 200433,China [3]Institute of Metabolism and Integrative Biology (IMIB),Key Laboratory of Reproduction Regulation of NPFPC,Fudan University,Shanghai 200433,China [4]Institutes of Biomedical Sciences,Fudan University,Shanghai 200433,China [5]Department of Laboratory Animal Science,Fudan University,Shanghai 200032,China

出　　处：《Genes & Diseases》2023年第2期317-320,共4页基因与疾病（英文）

基　　金：supported by grants from the State Key Development Programs of China(No.2018YFA0800300 to S-MZ);the National Natural Science Foundation of China(No.31821002,31930062 to S-MZ,81872373 to JY);the Shanghai Science and Technology Development Foundation(No.20ZR1404500 to YL);the Science and Technology Research Program of Shanghai(No.19DZ2282100 to HL).

摘　　要：Long noncoding RNAs(lncRNAs)are known to be involved in transcriptional regulation and their deregulation is associated with the development of human diseases such as cancer.1,2 LncRNA can directly bind to purine-rich double-stranded DNA sequences through Hoogsteen base pairing to form an RNA-DNA triplex motifs and regulate gene expression.3,4,5 However,its regulatory mechanisms and functions in tumors remain unclear.Here,we report that the LMNTD2 antisense RNA 1(LMNTD2-AS1,also known as AP006284.1)is highly expressed in prostate cancer(PCa)and positively correlated with the expression of its adjacent coding gene N-terminal Ras-association domain family 7(RASSF7).Mechanistically,AP006284.1 tethers to the RASSF7 promoter via RNA-DNA triplexes in cis-acting manner,which enhances chromatin accessibility and recruits the transcription factor complex GNL3/SFPQ to activate the expression of RASSF7,a repressor of the Hippo signaling pathway.Consistently,overexpression of either AP006284.1 or RASSF7 inactivated the Hippo signaling and malignant proliferation of PCa cells.

关 键 词：expression LncRNA cancer 

分 类 号：R737.25[医药卫生—肿瘤]