Single-cell analysis reveals the lncRNA-MEG3/miRNA-133a-3p/PRRT2 axis regulates skeletal muscle regeneration and myogenesis  

作　　者：Yilong Yao Zishuai Wang Yun Chen Lei Liu Liyuan Wang Guoqiang Yi Yalan Yang Dazhi Wang Kui Li Zhonglin Tang 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518000,China [2]Kunpeng Institute of Modern Agriculture at Foshan,Foshan,Guangdong 528226,China [3]Center for Regenerative Medicine University of South Florida Health Heart Institute,Morsani College of Medicine,University of South Florida,FL 33602,USA [4]Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518000,China [5]GuangXi Engineering Centre for Resource Development of Bama Xiang Pig,Bama,Guangxi 547500,China [6]State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China

出　　处：《Genes & Diseases》2023年第2期359-362,共4页基因与疾病（英文）

基　　金：supported by The National Natural Science Foundation of China(No.31830090);The Basic and Applied Basic Research Foundation of Guangdong province,China(No.2019B1515120059);The Agricultural Science and Technology Innovation Program,China(No.CAAS-ZDRW202006).

摘　　要：Skeletal muscle is the largest motor and metabolic organ of the body, which has a robust capacity for regeneration following injury or disease. Delayed regeneration after skeletal muscle injury reduces muscle contractility and leads to dysfunction of innervation. Therefore, identifying the regulation components in skeletal muscle regeneration and determining their molecular mechanisms are important to discover novel therapeutic markers for muscular diseases. Long non-coding RNA (LncRNA) has been implicated in skeletal muscle regeneration. Recent developed single-cell RNA sequencing (scRNA-seq) provides a higher resolution of cellular differences than bulk RNA-seq. Here, we re-analyzed single-cell transcriptomes data of skeletal muscle regeneration and identified lncRNA maternally expressed gene 3 (lncRNA-MEG3) was highly expressed in muscle satellite cells (MuSCs). Further study showed that lncRNA-MEG3 regulates skeletal muscle regeneration via sponging miR-133a-3p to regulate proline-rich transmembrane protein 2 (PRRT2) expression level. These results suggested that lncRNA-MEG3 might be a potential target for skeletal muscle diseases.

关 键 词：MEG3 SKELETAL MUSCULAR 

分 类 号：R68[医药卫生—骨科学]